polysacharin inhibits hepatocellular carcinoma-like phenotypes in a murine HCC model through repression of M2 polarization of tumour-associated macrophages.

Context: polysaccharin (APS), an extract of Schischk, exerts antitumor effects in hepatocellular carcinoma (HCC).

Objective: This study investigated the mechanism of action of APS in HCC.

Materials And Methods: Tumour-associated macrophages (TAMs) were treated with APS (0, 8, 16 mg/mL) for 24 h.

Protein sequence information extraction and subcellular localization prediction with gapped k-Mer method.

Background: Subcellular localization prediction of protein is an important component of bioinformatics, which has great importance for drug design and other applications. A multitude of computational tools for proteins subcellular location have been developed in the recent decades, however, existing methods differ in the protein sequence representation techniques and classification algorithms adopted.

Results: In this paper, we firstly introduce two kinds of protein sequences encoding schemes: dipeptide information with space and Gapped k-mer information.

Inhibition of Snail Family Transcriptional Repressor 2 (SNAI2) Enhances Multidrug Resistance of Hepatocellular Carcinoma Cells.

China accounts for almost half of the total number of liver cancer cases and deaths worldwide, and hepatocellular carcinoma (HCC) is the most primary liver cancer. Snail family transcriptional repressor 2 (SNAI2) is known as an epithelial to mesenchymal transition-inducing transcription factor that drives neoplastic epithelial cells into mesenchymal phenotype. However, the roles of endogenous SNAI2 remain controversial in different types of malignant tumors.

N,N'-di-(m-methylphenyi)-3,6-dimethyl-1,4-dihydro-1,2,4,5-tetrazine-1,4-dicarboamide (ZGDHu-1) suppresses the proliferation of PANC-1 pancreatic cancer cells via apoptosis and G2/M cell cycle arrest.

Pancreatic cancer is one of the human gastrointestinal malignancies with a high mortality and poor prognosis. Approximately eighty percent of patients are diagnosed with unresectable or metastatic disease. Thus, development of novel chemicals in the treatment of pancreatic cancer is imperative.

ZGDHu-1 induces G₂/M phase arrest and apoptosis in Kasumi-1 cells.

The present study examined the effects of N,N'‑di‑(m‑methylphenyi)‑3, 6‑dimethyl‑1, 4‑dihydro‑1,2,4,5‑tetrazine‑1,4‑dicarboamide (ZGDHu‑1), a novel oxazine derivative, in Kasumi‑1 cells. Following incubation with various concentrations of ZGDHu‑1, fluorescence‑activated cell sorting (FACS) was used in order to detect changes in mitochondrial membrane permeability in Kasumi‑1 cells. Western blot analysis was performed in order to analyze the expression of nuclear factor‑κB, inhibitor of κB and AML1/ETO.

Synthesis and antitumor activity of feruloyl and caffeoyl derivatives.

We developed two efficient protocols for the synthesis of feruloyl and caffeoyl derivatives from commercial vanillin and veratraldehyde. Pharmacological activities were assessed against a panel of human cancer cell lines in vitro. Most synthesized compounds demonstrated attractive cytotoxicity.