Publications by authors named "Ya-Ni Sun"
Article Synopsis
- Polymethyl methacrylate (PMMA) bone cement is widely used in orthopedic surgery but has limitations like shrinkage, heat injury, and low bioactivity.
- Researchers developed a new material, expandable P(MMA-AA-St), which reduces shrinkage but lacks strong biomechanical properties, leading to the addition of graphene oxide (GO) for improvement.
- The modified composite, PGBCs, showed enhanced strength, water absorption, and cell compatibility, with better outcomes in rabbit bone defect studies, indicating its potential as an effective biomaterial for implants.
Article Synopsis
- - The study focused on Hepatitis E virus (HEV) in swine, specifically examining bile and serum samples from a slaughterhouse and farms in Shandong province, China, to identify the virus’s genotypes and assess antibody prevalence.
- - Out of 106 bile samples tested, 30.2% were positive for HEV, with the majority belonging to genotype 4, and a complete genome sequence of subtype 4d was identified.
- - The results showed a 100% seroprevalence of HEV IgG antibodies among the herds and a significant presence of IgG (66.4%) in individual pigs, raising concerns about the risks of zoonosis and food safety regarding pork products.
Article Synopsis
- * The study aimed to investigate the antigenic domains in ORF3 of a specific avian HEV isolate by creating various antigens and using different antibodies from infected mice and chickens.
- * Researchers identified three key antigenic domains in ORF3, with one being particularly dominant, which is significant for developing diagnostic immunoassays for avian HEV infection.
The capsid protein (CP) of duck circovirus (DuCV) is the major immunogenic protein and has a high proportion of arginine residues concentrated at the N terminus of the protein, which inhibits efficient mRNA translation in prokaryotic expression systems. In this study, we investigated the subcellular distribution of DuCV CP expressed via recombinant baculoviruses in Sf9 cells and the DNA binding activities of the truncated recombinant DuCV CPs. The results showed that two independent bipartite nuclear localization signals (NLSs) situated at N-terminal 1-17 and 18-36 amino acid residue of the CP.
View Article and Find Full Text PDFDuck circovirus (DuCV) is classified in the genus Circovirus of the Circoviridae family. Two major open reading frames (ORFs), encoding the replicase (ORF1/rep) and the capsid protein (ORF2/cap), have been recognized for DuCV. Sequence analysis show that another major conserved ORF (named ORF3) is located in the complementary strand of ORF1/rep of DuCV, and its function remains to be investigated.
View Article and Find Full Text PDFAvian hepatitis E virus (HEV) has been identified in chickens; however, only 4 complete or near-complete genomic sequences have been reported. We found that the near-complete genomic sequence of avian HEV in chickens from China shared the highest identity (98.3%) with avian HEV from Europe and belonged to avian HEV genotype 3.
View Article and Find Full Text PDFObjective: To evaluate the combination of 7 interventional pulmonology methods in early diagnosis of lung cancer.
Methods: A total of 467 patients with thoracic and pulmonary lesions (include hilum pulmonis lymphadenectasis, mediastinal lymphadenectasis, pulmonary scobination, lump, lamellar infiltration, small amount of pleural fluid and pleural scobination) had negative results via exfoliative cytology, bacteriology and routine bronchoscopy. All these patients had ultrathin bronchoscopy with biopsy and brushing.
The clinical symptoms of infectious coryza are multiple and include nasal discharge, facial swelling, lacrimation, and anorexia. In general, the disease is not fatal to chicken; so, in experiments where animals are infected with Avibacterium paragallinarum, there have been debates about conducting the challenge model and evaluating the clinical signs. In this experiment, 150 chickens, aged 30 days, were randomly divided into different groups.
View Article and Find Full Text PDFThe genomic DNA extracted from chicken embryo fibroblasts (CEF) of SPF chickens from three chicken farms was used as template to amplify the ALV proviral DNA by PCR with four pairs of primers, high positive detection rates of gag - gene (29/46), pol - gene (27/46), env - gene (24/46) and LTR fragment (31/46) were achieved. Eight continuous and overlapping fragments were amplified from one DNA sample with 8 pairs of primers according to published sequences, then cloned into the TA vector and se quenced. The complete sequence of the whole genome of ALV strain SD0501 was established and analyzed with DNAstar software.
View Article and Find Full Text PDFAim: To investigate the apoptosis in esophageal cancer cells induced by resveratrol, and the relation between this apoptosis and expression of Bcl-2 and Bax.
Methods: In in vitro experiments, MTT assay was used to determine the cell growth inhibitory rate. Transmission electron microscope and TUNEL staining method were used to quantitatively and qualitively detect the apoptosis status of esophageal cancer cell line EC-9706 before and after the resveratrol treatment.