Application value research of swallowing treatment device combined with swallowing rehabilitation training in the treatment of swallowing disorders after stroke.

Background: Stroke is a common disabling disease, whether it is ischemic stroke or hemorrhagic stroke, both can result in neuronal damage, leading to various manifestations of neurological dysfunction.

Aim: To explore of the application value of swallowing treatment device combined with swallowing rehabilitation training in the treatment of swallowing disorders after stroke.

Methods: This study selected 86 patients with swallowing disorders after stroke admitted to our rehabilitation department from February 2022 to December 2023 as research subjects.

Growth of human endothelial cells on different concentrations of Gly-Arg-Gly-Asp grafted chitosan surface.

To improve the adhesion and growth of endothelial cells on chitosan, different concentrations of cell adhesive peptide, Gly-Arg-Gly-Asp (GRGD), were photochemically grafted to its surface. After different concentrations of GRGD-SANPAH (N-Succinimidyl-6-4'-azido-2'-nitrophenylamino]-hexanoate) solutions were formed, they were grafted to chitosan surfaces by performing surface adsorption of the peptide solutions and subsequent ultraviolet (UV) irradiation to induce photoreaction. The grafted efficiency for GRGD peptide to chitosan surface was higher than 80%, as semiquantitatively analyzed by a high performance liquid chromatography (HPLC).

Growth of human endothelial cells on photochemically grafted Gly-Arg-Gly-Asp (GRGD) chitosans.

To improve ECs adhesion and growth on chitosan, cell adhesive peptide Gly-Arg-Gly-Asp (GRGD) was photochemically grafted to its surface. Grafting 0.025 M of GRGD-SANPAH (N-Succinimidyl-6-[4'-azido-2'-nitrophenylamino]-hexanoate) solutions to chitosan and tripolyphosphate anhydrous crosslinked chitosan (chitosan-TPP) surfaces was performed by surface adsorption of the peptide and subsequent ultraviolet (UV) irradiation for photoreaction.