Publications by authors named "Ya-Fei Huang"
A series of 6-substituted carbazole-based retinoic acid-related orphan receptor gamma-t (RORγt) modulators were discovered through 6-position modification guided by insights from the crystallographic profiles of the "short" inverse agonist 6. With the increase in the size of the 6-position substituents, the "short" inverse agonist 6 first reversed its function to agonists and then to "long" inverse agonists. The cocrystal structures of RORγt complexed with the representative "short" inverse agonist 6 (PDB: 6LOB), the agonist 7d (PDB: 6LOA) and the "long" inverse agonist 7h (PDB: 6LO9) were revealed by X-ray analysis.
View Article and Find Full Text PDFBackground: The root-knot nematode Meloidogyne graminicola has become a serious threat to rice production as a result of the cultivation changes from transplanting to direct seeding. The nematicidal activity of Aspergillus welwitschiae have been investigated in vitro, and the disease control efficacy of the active compound has been evaluated under greenhouse and field conditions.
Results: The active compound αβ-dehydrocurvularin (αβ-DC), isolated by nematicidal assay-directed fractionation, showed significant nematicidal activity against M.
Objectives: To evaluate the classification and diameter of left gastric vein (LGV) in healthy Chinese adults with multi-detector computed tomography (MDCT).
Methods: MDCT angiography was performed in 234 healthy adults for the portal venous system. CT cross-sectional thin-layer reconstruction combined with maximum intensity projection, volume rendering and multiplanar reconstruction were applied.
Article Synopsis
- The study aimed to analyze the amino acids present in the fruit of Clausenae lansium.
- Researchers used HPLC techniques to measure both hydrolyzed and free amino acids in fresh and dried samples, employing specific mobile phases for accuracy.
- Results indicated the presence of 16 amino acids, including 10 essential ones, with total amino acid content of 4.8 mg/g and 15.0 mg/g for free and hydrolyzed amino acids respectively in both fresh and dried fruit.
Pretreatment with soluble ST2 reduces warm hepatic ischemia/reperfusion injury.
Biochem Biophys Res Commun
December 2006
The interleukin-1 receptor-like protein ST2 exists in both membrane-bound (ST2L) and soluble form (sST2). ST2L has been found to play an important regulatory role in Th2-type immune response, but the function of soluble form of ST2 remains to be elucidated. In this study, we report the protective effect of soluble ST2 on warm hepatic ischemia/reperfusion injury.
View Article and Find Full Text PDF[Study on chemical constituents of volatile oil and trace elements from fruits of Clausena lansium].
Zhongguo Zhong Yao Za Zhi
June 2006
Article Synopsis
- * Essential oils were extracted using supercritical fluid extraction, analyzed with techniques like GC-MS, and trace elements were determined through ICP-MS and ICP-AES.
- * 36 components made up 95% of the essential oil, with 18 compounds being newly discovered in these fruits.
Article Synopsis
- 4-1BB is a protein that helps activate T cells, and this study explored the effects of a specific gene therapy (Ad4-1BBIg) on heart transplant survival in mice.
- Using a heart transplant model, researchers injected either Ad4-1BBIg or a control vector (AdEGFP) into mice after they received a heart graft, monitoring T-cell activity and graft survival.
- Results showed that Ad4-1BBIg significantly prolonged heart graft survival (13.5 days vs. 7.5 days for control), reduced T-cell infiltration, and lowered levels of inflammatory markers, suggesting it could be a promising approach to prevent organ rejection.
[HLA-G1 molecule expressed by ECV304 cells inhibit cytotoxic activity of allogeneic NK cells].
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi
November 2005
Aim: To study the effect of HLA-G1 molecule expressed by an endothelial cell line (ECV304) on the cytotoxic activity of allogeneic NK cells.
Methods: ECV304 cells were transfected with recombinant plasmid pcDNA3-HLA-G1 by the liposome transfection, and the expressed HLA-G1 on the cell surface was detected by indirect immunofluorescent assay and flow cytometry. The cytotoxic activity of allogeneic NK cells against ECV304 cells was analyzed by the MTT method.
[In vitro refolding and functional study of soluble HLA-G1-peptide complex].
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi
May 2005
Aim: To form soluble HLA-G1-peptide complex by refolding in vitro, and to study its immune function.
Methods: The heavy chain and beta(2m) of sHLA-G1 were expressed as insoluble aggregates in E. coli, and then the two subunits were refolded to form HLA-G1-peptide complex by dilution method in the presence of specific peptide.
Objective: To investigate the immune tolerance inducing effects of soluble human leucocyte antigen G1 (sHLA-G1) on natural killer (NK) cells and T cells.
Methods: A recombinant plasmid expressing sHLA-G1 was constructed and transfected into human lymphoblastoid cells LCL721.221.
[Chemical components of essential oils from Flos chrysanthemi Indici in different areas].
Zhongguo Zhong Yao Za Zhi
April 2002
Objective: To provide the foundation for reasonable utilization by analysing the essential oils of Flos chrysanthemi Indici in different areas.
Method: The essential oils were extracted by using steam distillation and separated with GC capillary columns. The components were quantitatively determined with normalization method, and were identified with GC-MS.