Sensitivity-enhanced self-powered biosensing platform for detection of sugarcane smut using Mn-doped ZIF-67, RCA-DNA nano-grid array and capacitor.

Sugarcane smut is a widespread fungal disease, which severely impairs the quality and sugar yield of sugarcane. Early detection is crucial for mitigating its impact, which makes the development of a highly sensitive and accurate detection method essential. Herein, the Mn-doped zeolite imidazolate framework (ZIF-67), synthesized via a nano-confined-reactor approach, is designed to significantly enhance electron transport and boost the enzyme loading capacity within biofuel cells, thereby potentially enhancing their overall performance.

Dual-modal improved biosensing platform for sugarcane smut pathogen based on biological enzyme-Mg DNAzyme coupled with DNA transporter cascading hybridization chain reaction.

Sugarcane smut is a major disease affecting the yield and quality of sugarcane, and its early detection is crucial for the healthy development of sugarcane industry. In this work, a dual-modal biosensing platform is designed based on Au-V-MOF and 3D DNA walker for highly sensitive and precise detection of the sugarcane smut pathogen. This detection system utilizes the catalytic properties of biocatalysts and the precise cleavage of DNAzymes, along with 3D DNA walker nanotechnology and a designed "walking track", to achieve amplified detection signals and accurate target recognition.

Super signal-enhancement biosensing platform for precise target recognition based on rolling circle-hybridization chain dual linear cascade amplification technology.

This paper presents a self-powered biosensing platform based on graphdiyne@Au (2D GDY@Au) nanoparticles and rolling circle-hybridization chain (RC-HC) dual linear cascade amplification technology, which significantly enhances target recognition and signal amplification efficiency for miRNA-141. Specifically, the target on bioanode outputs a large amount of single-stranded DNA (T1) through the strand displacement amplification (SDA) mechanism. This efficient target recycling process triggers RC-HC dual linear cascade reaction.

Entropy-driven self-assembled enzyme-DNA nanomatrix cascade DNAzyme-CRISPR/cas system for multiplexed enhancement of self-powered sensing platform for protein detection.

Clustered Regularly Interspaced Short Palindromic Repeats and CRISPR-associated Proteins (CRISPR/Cas) system can accurately identify and cleave target DNA sequences, while the effective combination of DNA nanomatrix and entropy-driven self-assembled enzymes can significantly enhance the sensitivity, stability, and diversified functionality of sensors through highly ordered molecular arrangement and spontaneous efficient assembly processes. Herein, a carbon-encapsulated MoS hollow nanorod (C-MoS) with excellent conductivity and multiple active sites is used to construct bioanode of biofuel cell by integrating it with an entropy-driven self-assembled enzyme-DNA nanomatrix cascade DNAzyme-CRISPR/Cas system. When thrombin binds aptamer, it exposes the trigger strand on the anode, initiating chain displacement.

A dual-mode strategy for early detection of sugarcane pokkah boeng disease pathogen: A portable sensing device based on Cross-N DNA framework and MoS@GDY.

Sucrose, a common sugar primarily derived from sugarcane, is a crucial national strategic resource. However, its yield is significantly affected by various serious diseases, with pokkah boeng disease being one of the most damaging. Therefore, developing a sensitive method for the accurate detection of the pokkah boeng disease pathogen is crucial for ensuring the safety of sugar.

The impact of China's digital inclusive financial development gap on the optimization of rural consumption structure.

Implementing the rural revitalization strategy is crucial for ensuring and enhancing the livelihoods of the vast rural population. The upgrading of rural consumption reflects the gradual realization of rural residents' pursuit of a better life, and the rapid development of digital inclusive finance provides strong support for this. Based on the Digital Inclusive Finance Index released by Peking University and panel data from 30 provinces across the country, this study examines the role of digital inclusive finance in optimizing rural consumption structure through the mediation effect model and analyzes its spatial spillover effects using the spatial Durbin model.

Multiple signal amplification strategy induced by biomarkers of lung cancer: A self-powered biosensing platform adapted for smartphones.

Lung cancer is a major malignant cancer with low survival rates, and early diagnosis is crucial for effective treatment. Herein, a biosensing platform that is self-powered derived from a capacitor-coupled EBFC has been developed for ultra-sensitive real-time identification of microRNA-21 (miRNA-21) with the assistance of a mobile phone. The flexible substrate of the platform is prepared on a carbon paper modified with graphdiyne and gold nanoparticles.

Digital inspection techniques of modular integrated construction.

As a new construction form, modular integrated construction (MiC) can effectively improve the construction quality and productivity, especially for the construction of high-density and high-rise buildings. However, the current MiC quality inspection relies on manual inspection, which is inefficient and unreliable. Systematic research on digital inspection techniques (DITs) is fragmented and unable to fully realize the potential of the MiC industry.

An electrochemical aptasensor for detection of carbofuran using gold nanoparticles decorated hierarchical porous carbon as an effective sensing platform.

In this study, a novel electrochemical aptasensor for carbofuran (CBF) detection is prepared by gold nanoparticles decorated hierarchical porous carbon (Au@HPC). The prepared carbon materials show a three-dimensional hierarchical structure with a large specific surface area and a highly developed porous structure. Aptamers loading significantly improves when gold nanoparticles are embedded into the hierarchical porous carbon skeleton.

A novel electrochemical aptasensor based on polyaniline and gold nanoparticles for ultrasensitive and selective detection of ascorbic acid.

Ascorbic acid (AA) is involved in many physiological activities of the body and plays an important role in maintaining and promoting human health. It is also present in many natural and artificial foods. Therefore, the development of highly sensitive and accurate AA sensors is highly desirable for human health monitoring, as well as other commercial application fields.

Comparison of the clinical efficacy of donafinil and lenvatinib in the treatment of intermediate and advanced hepatocellular carcinoma.

Objective: To compare the therapeutic effects of donafinil and lenvatinib in the treatment of patients with intermediate--advanced hepatocellular carcinoma (HCC).

Methods: A total of 100 patients with intermediate--advanced HCC who received donafinib or lenvatinib treatment in Hechi First People's Hospital, Hechi People's Hospital, the Second Affiliated Hospital of Guangxi University of Science and Technology, and other centers from January 2021 to June 2022 were retrospectively analyzed. The patients were classified into a donafinil group (n=50) and a lenvatinib group (n=50) according to the treatment method.

A carbon dot-based nanoscale covalent organic framework as a new emitter combined with a CRISPR/Cas12a-mediated electrochemiluminescence biosensor for ultrasensitive detection of bisphenol A.

Exploring new highly efficient electrochemiluminescence (ECL) luminophores is a necessary condition for developing ultrasensitive ECL biosensors. Therefore, a luminescent carbon dot-based covalent organic framework (CD-COF) was prepared using aldehyde-based carbon dots (CDs) and 1,3,5-tris (4-aminophenyl) benzene (TPB). Because the CD-COF made the regular arrangement of CDs conducive to improving the ECL response, CD-COF had a higher ECL intensity and efficiency than CDs.

Highly sensitive electrochemical detection of myricetin in food samples based on the enhancement effect of Al-MOFs.

Microporous aluminum-based metal-organic frameworks (CAU-1) are used to develop a simple and sensitive electrochemical sensor for myricetin (MYR) based on a modified carbon paste electrode (CPE) for the first time. The morphologies and electrochemical properties of the as-synthesized CAU-1 are studied utilizing various analytical methods including scanning electron microscopy, transmission electron microscopy, X-ray diffraction, Fourier transform infrared spectroscopy, N adsorption-desorption, and electrochemical impedance spectroscopy. In terms of electrochemical oxidation of MYR, CAU-1/CPE with its large number of active micropores and rapid electron transfer demonstrates superior performance compared to the bare CPE.

A Miniature Soft Sensor with Origami-Inspired Self-Folding Parallel Mechanism.

Miniature soft sensors are crucial for the perception of soft robots. Although centimeter-scale sensors have been well developed, very few works addressed millimeter-scale, three-dimensional-shaped soft sensors capable of measuring multi-axis forces. In this work, we developed a millimeter-scale (overall size of 6 mm × 11 mm × 11 mm) soft sensor based on liquid metal printing technology and self-folding origami parallel mechanism.

Machine Learning Models for Diagnosis of Parkinson's Disease Using Multiple Structural Magnetic Resonance Imaging Features.

Purpose: This study aimed to develop machine learning models for the diagnosis of Parkinson's disease (PD) using multiple structural magnetic resonance imaging (MRI) features and validate their performance.

Methods: Brain structural MRI scans of 60 patients with PD and 56 normal controls (NCs) were enrolled as development dataset and 69 patients with PD and 71 NCs from Parkinson's Progression Markers Initiative (PPMI) dataset as independent test dataset. First, multiple structural MRI features were extracted from cerebellar, subcortical, and cortical regions of the brain.

[Differential diagnosis of pure ground glass nodules in lung with spectral CT imaging].

To evaluate the differential diagnostic performance of quantitative parameters derived from the spectral CT imagingin pure ground-glass nodules. A total of 44 patients with pure ground glass nodules underwent chest energy spectrum CT and with known subsequently pathological findings in the Imaging Department of the Second Affiliated Hospital of Soochow University from August 2017 to September 2019 were retrospectively analyzed. Among them, there are 18 males and 26 females, aged from 26 to 79 (51±12) years.

Tumor-derived exosomal BCYRN1 activates WNT5A/VEGF-C/VEGFR3 feedforward loop to drive lymphatic metastasis of bladder cancer.

Background: Patients with lymph node (LN) metastatic bladder cancer (BCa) present with extremely poor prognosis. BCa-derived exosomes function as crucial bioactive cargo carriers to mediate the signal transduction in tumor microenvironment triggering tumor metastasis. However, the mechanisms underlying exosome-mediated LN metastasis in BCa are unclear.

Reflections on the aconitine poisoning.

Aconitine is a natural toxic substance belonging to the family of aconitum alkaloids, which is used in small doses by Traditional Chinese practitioners. We present a case of accidental death due to acute aconitine poisoning. A 65-year-old Chinese man presented to a medical clinic with fever and shoulder pain.

Patterns of Sulcal depth and cortical thickness in Parkinson's disease.

Previous voxel-based morphometry (VBM) and cortical thickness (CT) studies on Parkinson's disease (PD) have mainly reported the gray matter size reduction, whereas the shape of cortical surface can also change in PD patients. For the first time, we analyzed sulcal depth (SD) patterns in PD patients by using whole brain region of interest (ROI)-based approach. In a cross-sectional study, high-resolution brain structural MRI images were collected from 60 PD patients without dementia and 56 age-and sex-matched healthy controls (HC).

Disulfiram-copper activates chloride currents and induces apoptosis with tyrosine kinase in prostate cancer cells.

Aim: To elucidates the mechanism that disulfiram/copper complex (DSF/Cu) treatment activates chloride channels and induces apoptosis in prostate cancer cells.

Methods: Cellular membrane currents were measured by membrane clamp technique; western blot to detect protein expression; flow cytometry to detect apoptosis; immunofluorescence to detect target protein co-localization, and further validated by a combination of protein-protein interaction and mock protein molecular docking techniques.

Results: DSF/Cu activated chloride channels and induced apoptosis in LNCaP (a type of androgen-dependent prostate cancer cells) cells.

High-performance electrochemical sensing platform based on sodium alginate-derived 3D hierarchically porous carbon for simultaneous determination of dihydroxybenzene isomers.

Three-dimensional hierarchically porous carbon (denoted as SA-900) with a microporous, mesoporous and macroporous structure was facilely fabricated via direct carbonization of sodium alginate. SA-900 was fully characterized by N adsorption-desorption, scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-ray diffraction and Raman spectroscopy to confirm its structure. SA-900 was coated onto a glassy carbon electrode surface to construct an ultrasensitive electrochemical sensing platform (SA-900/GCE).

Abnormal Topological Organization of Sulcal Depth-Based Structural Covariance Networks in Parkinson's Disease.

Recent research on Parkinson's disease (PD) has demonstrated the topological abnormalities of structural covariance networks (SCNs) using various morphometric features from structural magnetic resonance images (sMRI). However, the sulcal depth (SD)-based SCNs have not been investigated. In this study, we used SD to investigate the topological alterations of SCNs in 60 PD patients and 56 age- and gender-matched healthy controls (HC).

[Molecular cloning and characterization of a novel metagenome-derived bacterial perhydrolase].

The aim of this study is to obtain bacterial perhydrolases with chlorination activity, expands the resources of perhydrolases, and lays a foundation for it's industrial applications. We constructed a metagenomic library using environmental DNA isolated from sludge samples of a paper mill of Tanghe county, and identified a per822 gene encoding a bacteria perhydrolase via activity-based functional screening. Then, we overexpressed Per822 heterologously in Escherichia coli, and characterized the recombinant enzyme after purification.