Benzoic acid facilitates ANF in monocot crops by recruiting nitrogen-fixing Paraburkholderia.

Associative nitrogen fixation contributes large portion of N input to agro-ecosystems through monocot-diazotrophic associations. However, the contribution of associative nitrogen fixation is usually neglected in modern agriculture, and the underlying mechanisms of association between monocot and diazotrophs remain elusive. Here, we demonstrated that monocot crops employ mucilage and associated benzoic acid to specially enrich diazotrophic partners in response to nitrogen deficiency, which could be used for enhancing associative nitrogen fixation in monocot crops.

Genetic analysis and fine mapping of phosphorus efficiency locus 1 (PE1) in soybean.

PE1 is a valuable locus synergistically regulating both P acquisition and utilization efficiency. Phosphorus (P) is required for crop production, particularly for soybean, due to its high protein and oil contents, and large demands for P in biological nitrogen fixation. Therefore, enhancing P efficiency is a practical and important target for soybean-breeding programs; however, the genetic mechanisms are still unclear.

[Effect of planting white clover on nutrients and biological properties of soils in persimmon orchard of Weibei, Shaanxi Province, China].

Leguminous crop planting is one of the most important managements in sustainable agriculture, which has the potential to improve soil quality and environmental health by increasing N input and facilitating soil and water conservation. In this study, effects of white clover planting on soil nutrients and biological properties in persimmon orchard was investigated to understand its potential effects on improvement of soil fertility and economic performance. Soil samples were collected on September 14, 2017 at 0-10 cm, 10-20 cm, 20-30 cm and 30-40 cm soil depth in two treatments (i.

Identification and Mapping of a New Soybean Male-Sterile Gene, .

The use of sterility is common in plants and multiple loci for hybrid sterility have been identified in crops such as rice. In soybean, fine-mapping and research on the molecular mechanism of male sterility is limited. Here, we identified a male-sterile soybean line, which produces larger, abnormal pollen grains that stain poorly with I-KI.

Ridge-furrow with plastic film and straw mulch increases water availability and wheat production on the Loess Plateau.

Mulching is critical for increasing water availability and hence winter wheat production in dryland farming systems. A two-year study was conducted to assess the effects of mulches on soil water storage (SWS), temperature, water use efficiency (WUE) and yields of winter wheat on the Loess Plateau. Four treatments were examined: conventional flat planting (CK), straw mulch (FPS), transparent plastic film mulch (FPP) and ridge-furrow with plastic film-mulched ridge and straw-mulched furrow (RFPS).

Successful treatment of an acute infective endocarditis secondary to fish bone penetrating into left atrium caused by Granulicatella adiacens and Candida albicans: A case report.

Rational: Infective endocarditis caused by a foreign body of the upper digestive tract is rare. We report a rare case of Granulicatella adiacens and Candida albicans coinfection acute endocarditis combined with systematic embolization caused by a fish bone from the esophagus penetrating into the left atrium.

Patient Concern: A 42-year-old woman was admitted to our hospital because of fever, abdominal pain, headache, and right limb weakness.

[Effects of reducing N, straw returning and dicyandiamide application on winter wheat yield and nitrogen budgets in rain-fed region.].

In a 3-year field experiment, effects of reducing N fertilizer, straw returning and dicyandiamide (DCD) application on wheat yield and nitrogen budgets under wheat-fallow system were studied in rain-fed region. The experiment was carried out in Yangling of Shaanxi Province, and 4 treatments were installed including no nitrogen (CK), 220 kg N·hm and no straw returning (FP), 150 kg N·hm and straw returning (OPT), 150 kg N·hm, 7.5 kg·hm DCD and straw returning (OPT+DCD).

[Present situation of rice fertilization in qin-ba mountainous area of Southern Shaanxi, China].

In order to understand the present situation of rice fertilization and the existing problems in the farmers' nutrient resources input in the Qin-Ba mountainous area of southern Shaanxi, the survey data from 2854 households in 11 counties of this area in the project "soil testing and formulated fertilization in 2006-2009" were analyzed and evaluated, and the countermeasures for the existing problems in the farmers' nutrient resources input were proposed. In the study area, the average rice yield was 7822 kg x hm(-2) per year, and the ratio of the households obtained the medium level yield was up to 50.9%.

[Effects of different long-term fertilization patterns on Fuji apple yield, quality, and soil fertility on Weibei Dryland, Shaanxi Province of Northwest China].

A 7-year (2003-2010) located field experiment was conducted to evaluate the effects of different long-term fertilization patterns on the Fuji apple yield, quality, and soil fertility on the Weibei Dryland, Shaanxi Province of Northwest China. Seven treatments were installed, i. e.

Genome-wide analysis reveals diversity of rice intronic miRNAs in sequence structure, biogenesis and function.

Intronic microRNAs (in-miRNAs) as a class of miRNA family that regulates gene expression are still poorly understood in plants. In this study, we systematically identified rice in-miRNAs by re-mining eight published small RNA-sequencing datasets of rice. Furthermore, based on the collected expression, annotation, and putative target data, we investigated the structures, potential functions, and expression features of these in-miRNAs and the expression patterns of their host genes.

MicroRNA profiles and their control of male gametophyte development in rice.

Plant microRNAs (miRNAs) act as negative regulators of gene expression by slicing target transcripts or inhibiting translation. A number of miRNAs play important roles in development. In order to investigate the potential function of miRNAs during male gametogenesis in rice, we obtained both gene and small RNA expression profiles by combining microarray and high-throughput sequencing technologies.

[Numerical evaluation of soil quality under different conservation tillage patterns].

A 9-year field experiment was conducted on the Guanzhong Plain of Shaanxi Province to study the effects of subsoiling, rotary tillage, straw return, no-till seeding, and traditional tillage on the soil physical and chemical properties and the grain yield in a winter wheat-summer maize rotation system, and a comprehensive evaluation was made on the soil quality under these tillage patterns by the method of principal components analysis (PCA). Comparing with traditional tillage, all the conservation tillage patterns improved soil fertility quality and soil physical properties. Under conservative tillage, the activities of soil urease and alkaline phosphatase increased significantly, soil quality index increased by 19.

[Nitrate leaching characteristics of wheat-corn rotation farmland in Guanzhong area of Shaanxi].

By using in situ leaching device, a field experiment was conducted to study the effects of nitrogen fertilization rate and straw mulching on the nitrate leaching at 90 cm soil depth, nitrate accumulation in soil profile (0-100 cm), crop yield, and nitrogen balance of wheat-corn rotation farmland in Guanzhong area of Shaanxi. Six treatments were installed, i. e.

Clinical features at the onset of childhood type 1 diabetes mellitus in Shenyang, China.

Aim: To describe the clinical picture and laboratory features of Chinese children with newly diagnosed type 1 diabetes mellitus.

Methods: The clinical and laboratory data of a total of 203 children who presented with newly diagnosed type 1 diabetes mellitus during a 5-year period (2004-2008) were retrospectively analysed based on hospital records.

Results: There were 88 boys (43.

[Atmospheric dry and wet nitrogen deposition in typical agricultural areas of North Shaanxi].

To investigate the farmland soil nitrogen input from atmospheric dry and wet deposition, a 1-year observation was conducted in the Yulin and Luochuan areas of North Shaanxi Province from June 2007 to May 2008. The total inorganic nitrogen (TIN) deposition in Yulin and Luochuan was 22.17 and 16.

[Weed biodiversity in winter wheat field of loess soil under different fertilization regimes].

Employing an inverted 'W' investigation procedure with 9 sampling locations and adopting a biodiversity analysis approach integrated with typical statistic method, this paper studied the effects of different long-term stationary fertilization regimes on the weed biodiversity in winter wheat fields on loess soil. The results showed that in the experimental plots, there were 16 weed species belonging to 10 family and 16 genera, occupying about 34% of the total number of weed species in winter wheat fields in Shaanxi Province. The weed biodiversity was decreased with the improvement of soil nutrient status.

Mutations of the p16 gene product are rare in prostate cancer.

Background: The p16 gene product is a negative regulator of cell cycle and has been shown to be deleted or mutated in a number of tumor cell lines and primary tumors. The role of p16 in prostate cancer is not defined. Prostate cancer tissues and cell lines were evaluated for p16 gene alterations.

Recombinant adenovirus vector expressing wild-type p53 is a potent inhibitor of prostate cancer cell proliferation.

Objectives: A recombinant adenovirus vector (AdWTp53) expressing wild-type p53 was evaluated for its cell growth inhibitory effects on metastatic human prostate cancer cells.

Methods: Human prostate cancer cells LNCaP, DU145, PC3, 1LN, and DUPro-1 were infected with AdWTp53 vector and expression of exogenous p53 in these cells was analyzed by immunoprecipitation and western blot assays. The cell growth inhibitory effects of AdWTp53 were determined by counting cell number on a hemocytometer or by crystal violet staining of cells after infection with AdWTp53.

Increased p53 protein does not correlate to p53 gene mutations in microdissected human testicular germ cell tumors.

Purpose: To determine if primary testicular germ cell tumors that overexpress p53 tumor suppressor gene protein have p53 gene mutations.

Materials And Methods: We examined 30 primary testicular tissues from 26 patients representing two groups. Group one consisted of eleven cases (6 nonseminomatous germ cell tumors and 5 seminomas) in which tissue samples for DNA analysis were microdissected from paraffin block regions with elevated immunohistochemical staining for p53 protein.

Dominant negative effect of a germ-line mutant p53: a step fostering tumorigenesis.

Lysates derived from the fibroblasts of individuals who are homozygous for normal p53 or heterozygous for the germ-line p53 mutation characteristic of certain Li-Fraumeni cancer-prone families were assessed for p53 function utilizing the binding of p53 protein to a p53-specific consensus oligonucleotide sequence. As expected, control nuclear lysates containing only mutant p53 or no p53 displayed little or no such binding. However, the nuclear lysates from heterozygous fibroblasts containing similar amounts of normal p53 and 245D mutant p53 displayed binding that was significantly below 50% of that seen with homozygous wild-type p53 in normal cell lysates.

Several mutant p53 proteins detected in cancer-prone families with Li-Fraumeni syndrome exhibit transdominant effects on the biochemical properties of the wild-type p53.

The identification of germ-line mutations in the p53 gene has provided a situation where comparable amounts of wild-type and mutant p53 co-exist in constitutional cells of certain individuals who are cancer-prone. Here we report the biochemical characteristics of several Li-Fraumeni syndrome associated mutant p53 proteins in order to assess the influence of germ-line mutant p53 on the functions of the wild-type p53. Unlike 248W mutant p53 protein, which was previously shown to have no effect on the wild-type p53 conformation (Milner & Medcalf, 1991; Cell 65, 765-774), germ-line associated mutant p53 proteins with residue 133T, 245D or 258K, converted the wild-type p53 conformation into the mutant conformation.

Oncogene- transformed NIH 3T3 cells display radiation resistance levels indicative of a signal transduction pathway leading to the radiation-resistant phenotype.

Oncogenes and their normal counterparts, proto-oncogenes, are functionally important cellular genes which interact with one another as components of signal transduction pathways leading to cell growth and differentiation. Numerous reports in the literature have also begun to link these genes to the phenomenon of cellular radiation resistance. In this report we examine the radiation resistance level of NIH 3T3 cells transformed by various oncogenes in an attempt to define the intracellular pathway to the radiation-resistant phenotype.