Publications by authors named "Ya Feng Qiu"
Mx proteins are interferon-induced GTPases that have broad antiviral activity against a wide range of RNA and DNA viruses. We previously demonstrated that porcine Mx1 protein (poMx1) inhibited the replication of classical swine fever virus (CSFV), an economically important Pestivirus, and that mouse Mx1 did so as well. It is unknown why the nucleus-localizing mouse Mx1 inhibits CSFV replication which occurs in the cytoplasm.
View Article and Find Full Text PDFAntiviral activity of doxycycline against vesicular stomatitis virus in vitro.
FEMS Microbiol Lett
November 2015
Doxycycline (Dox) is a tetracycline derivative with broad-spectrum antimicrobial activities that is used as an effector substance in inducible gene-expression systems. We investigated the antiviral activity of Dox against vesicular stomatitis virus (VSV) infection in cultured H1299 cells. Dox at concentrations of 1.
View Article and Find Full Text PDFThe bursa of Fabricius (BF) is the acknowledged central humoral immune organ in birds. Bursal septpeptide II (BSP-II) is an immunomodulatory bioactive peptide isolated from BF. To understand the effects of BSP-II on immune induction, gene expression profiles of hybridoma cells treated with BSP-II were evaluated.
View Article and Find Full Text PDFArticle Synopsis
- The bursa of Fabricius is crucial for B lymphocyte development and has potential antitumor activity through its derived peptides, specifically the newly identified BPP-II (MTLTG).
- BPP-II has been shown to influence a wide array of gene expressions related to immune responses, enhancing specific antibody and cytokine production in the presence of avian influenza.
- The peptide also promotes p53 expression and inhibits tumor cell growth, suggesting its role as an important factor in connecting the immune system and cancer therapy, paving the way for new immunotherapeutic strategies.
Glycyrrhetinic acid (GA) is an active component of licorice root that has long been used as a herbal medicine for the treatment of peptic ulcer, hepatitis, and pulmonary and skin diseases in Asia and Europe. In this study, we analyzed the effect of GA extracted from Glycyrrhiza uralensis Fisch. on the expression of Toll-like receptors (TLRs) that play key roles in regulating the innate immune response against invading pathogens.
View Article and Find Full Text PDFHsp70 potentiates specific immune responses to some antigenic peptides fused to it. A recombinant hsp70 protein expression vector in methylotrophic yeast, Pichia pastoris, was developed that fused the major antigenic segment of Japanese encephalitis virus (JEV) E protein to the amino terminus of Mycobacterium tuberculosis hsp70. The C-terminal peptide binding domain of hsp70 stimulated Th1-polarizing cytokines, CC chemokines and an adjuvant effect.
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- - The study aimed to enhance the efficiency of recombinant Marek's disease viruses (MDV) in chickens by selecting optimal promoters for constructing these viruses, with a focus on those that could work well even with maternal antibodies present.
- - Researchers created novel composite promoters by combining the MDV gB core promoter with regulatory elements from human cytomegalovirus (hCMV) and SV40, leading to the creation of eukaryotic expression vectors named PhCMV-gB, Psv-gB, and Pen-gB.
- - Testing showed that these composite promoters significantly improved gene expression compared to the full-length gB promoter, with PhCMV-gB being particularly effective, although they still had lower transcriptional activity
Article Synopsis
- A recombinant vector combining the JEV E protein with M. tuberculosis hsp70 was developed in yeast for easier protein expression and purification, avoiding common issues found in bacterial systems.
- Immunization studies in mice showed that the E-hsp70 fusion protein generated a stronger immune response compared to the individual components, as measured by cytokine production and antibody levels.