[Effects of simvastatin on homocysteine-induced endothelial dysfunction and inflammatory response].

Objective: To investigate the effects of simvastatin (SIM) on homocysteine (HCY)-induced endothelial dysfunction and inflammatory response.

Methods: Human umbilical vein endothelial cells (HUVECs) were isolated from the umbilical cords from healthy lying-in women and cultured and added with HCY of the concentrations of 0.1, 0.

[Studies on the reaction of balofloxacin with bovine serum albumin].

In the present paper, a fluorescence method was used to study at different pH the fluorescence quenching of bovine serum albumin (BSA) by its interaction with balofloxacin (BLFX). The interaction association constants of BSA and BLFX were determined from a double reciprocal line Weaver-Burk plot. According to the Forster dipole-dipole energy transfer, the distance to be measured between the BLFX and tryptophane is 5.

[Correlation studies of contents of copper and organic components in Astragalus roots].

Objective: To study the relationship between copper and organic components, total flavonoids of Astragalus (TFA), total saponins of Astragalus (TSA) and total polysaccharide of Astragalus (TPA).

Method: TFA, TSA and TPA were extracted from Astragalus roots using different organic solvents, and determined by colorimetry. The concentration of copper in extracts was determined by graphite furnace atomic absorption spectrometry (GFAAS).

Sequential uniform designs for fingerprints development of Ginkgo biloba extracts by capillary electrophoresis.

A sequential procedure for the method of development of fingerprints based on a uniform design approach has been described in this paper. The sequential uniform design is used to reach the global optimum for a separation. The procedure is illustrated through developing the fingerprint of a Ginkgo biloba extract by capillary electrophoresis (CE) with diode array detection (DAD).

[In vitro metabolic interaction between diphenytriazol and steroid hormone drugs].

Aim: To observe the metabolic interaction between diphenytriazol and steroid hormone drugs, and provide some useful information for clinical medication.

Methods: The steroid hormone drugs which may be co-administrated with diphenytriazol were selected, such as mifepriston, estradiol, medroxyprogesterone acetate, progesterone, norethisterone and so on. Diphenytriazol was incubated with each drug in rat liver microsome.

[Synthesis and AchE inhibitory activity of 2-phenoxy-indan-1-one derivatives].

Aim: To design and synthesize novel AchE inhibitors.

Methods: The condensation of 2-bromo-5, 6-dimethoxy-indan-1-one with various aminoalkyl phenols in the presence of K2CO3 and acetonitrile gave the corresponding title compounds, and the in vitro AchE and BchE inhibitory activities were evaluated by the modified Ellman method.

Results: Sixteen novel target compounds 8a - p were synthesized, their structures were confirmed by 1H NMR, MS, IR and elemental analysis.

[Synthesis and vasorelaxation action of flavonoids].

Aim: To search for flavonoids which possess stronger vasorelaxation action.

Methods: Four quercetin glycosides (1a - d) were synthesized from quercetin in three steps i. e.

Gender differences in limonin pharmacokinetics in rats.

In the present study, the pharmacokinetics of limonin (LM) were investigated in male and female rats. LM concentrations in the plasma were determined after the oral administration of 36 mg/kg LM or after intravenous (i.v.

[Inhibition of rat corneal neovascularization by inhibitor of nuclear factor-kappaB].

Objective: To observe the effects and mechanism of pyrrolidine dithiocarbamate (PDTC) on inhibiting corneal neovascularization.

Methods: The corneal neovascularization was induced by alkali burn in 48 Sprague-Dawley rats. The rats were randomly divided into 4 groups, 12 rats in each group.

[Synthesis and antitumor activity of arylsubstituted imidazolin-2-one derivatives].

Aim: To design and synthesize new arylsubstituted imidazolin-2-one analogues as antitumor compounds.

Methods: Arylsubstituted imidazolin-2-ones were prepared by condensation the appropriate omega-amino-acetophenone hydrochloride with arylisocyanate in toluene. The target compounds prepared in this study were tested for cytotoxicity against PC-3, A549, HO-8910, Hela, HL60, K562 and HL60R cancer cell lines, and mechanism of one of the products 4y was further evaluated with its mechanium.

[The ultrastructure change of cardiomyocyte in Athlete's Heart].

Objective: This study is to see the pathologic change of cardiac myocyte in Athlete's Heart, and explore the mechanism of the pathologic change.

Methods: Fifteen male SD rats were separated randomly into control group (without any exercise), aerobic exercise group (Ae group, swimming for 75 min every day), and overloading exercise group (Oe group, swimming for 180 min with a loading of 5 percent of body weight every day). After 5 days per week for 12 weeks, swimming stopped, the rat hearts were prepared to specimens and examined under Transmission Electron Microscope.

Determination of limonin in rat plasma by liquid chromatography-electrospray mass spectrometry.

A simple, sensitive and selective liquid chromatography coupled with electrospray ionization mass spectrometry (LC/ESI/MS) method for determination of limonin (LM) in rat plasma has been developed and validated. The method had advantages of a single liquid-liquid extraction with ether and high sensitivity. Analyses were conducted at a flow rate of 0.

Inhibition of hypoxia-induced proliferation and collagen synthesis by vasonatrin peptide in cultured rat pulmonary artery smooth muscle cells.

The aim of the present research is to investigate the effects of vasonatrin peptide (VNP) on hypoxia-induced proliferation and collagen synthesis in pulmonary artery smooth muscle cells (PASMCs). Smooth muscle cells isolated from rat pulmonary artery were cultured and used at passages 3-5. Cell proliferation and collagen synthesis were evaluated by cell counts, [(3)H] thymidine and [(3)H] proline incorporation.

Development, optimization and validation of a fingerprint of Ginkgo biloba extracts by high-performance liquid chromatography.

A four-step development, optimization and validation strategy for high-performance liquid chromatography (HPLC) fingerprints of Ginkgo biloba extract is described. A suitable chromatographic system was selected first. The following step was performing a screening design to select important parameters.

[The ultrastructure change of heart capillary in athlete's heart.].

Objective: To see the change of capillary of heart in Athlete's Heart, so that to discover the mechanism of pathologic change.

Method: 18 male SD rats were separated randomly into control group (without any exercise), aerobic exercise group (swimming for 75 min every day), and overload group (swimming for 180 min with 5% weight of its body every day). After 5 days per week, 12 weeks, exercise training stopped and heart of rats were observed under Transmission Electron Microscope.

[Study on ultrasonic extraction techniques of flavonoids in Chrysanthemum morifolium].

Objective: To optimize the ultrasonic extraction condition for flavonoids from Chrysanthemum morifolium.

Method: The extraction rate of flavonoids optimized condition (ethanol concentration, ultrasonic time, solvent quantity and extraction times) was determined by orthogonal design. UV-Spectrophotometry was used for the determination.

A luminescent Pt(II) complex with a terpyridine-like ligand involving a six-membered chelate ring.

The ligand 2-(8'-quinolinyl)-1,10-phenanthroline (1) was prepared in 79% yield by the Friedlander condensation of 8-amino-7-quinolinecarbaldehyde and 8-acetylquinoline. The complex [Pt(1)Cl]+ was prepared and compared with the isomeric 2-(2'-quinolinyl)-1,10-phenanthroline (2) complex. An X-ray analysis indicated that the six-membered chelate ring in the tridentate complex resulted in a relief of angle strain as well as some non-planarity in the bound ligand 1.

[Study on extraction technology for extract and flavonoids in Chrysanthemum morifolium by orthogonal design].

Objective: To ascertain extraction technology condition for extract and flavonoids from Chrysanthum morifoliwn.

Method: The optimizing ultrasonic extraction condition on the basis of extractive yield and flavonoids were determined by orthogonal design.

Result: The order of factors which affected the flavonoid extraction was extraction times > ethanol concentration > ultrasonic time > solvent quantity.

[Pathologic diagnosis of non-Alzheimer type dementia].

Objective: To characterize histopathologic features of non-Alzheimer type dementia.

Methods: Bodian, Gallyas-Braak silver staining, tau and ubiquitin immunohistochemistry were applied in an analysis of 22 cases of autopsy-proven neurodegenerative dementia. Appearance, distribution and immunoreactivity of neuronal and glial inclusions in the brain were observed.

[Endoscopic thyroidectomy with 150 cases].

Objective: To discuss the method, the advantages and disadvantages of endoscopic thyroidectomy.

Methods: Endoscopic thyroidectomy via areola of breasts approach was performed in 150 patients, including 41 cases of thyroid adenoma, 64 cases of nodular goiter, 40 cases of Graves' disease, and 5 cases of thyroid carcinoma.

Results: The endoscopic thyroidectomy was successfully carried out in 144 cases, including tumor dissection in 32 cases, one lobe partial thyroidectomy in 54 cases, two lobe partial thyroidectomy in 19 cases, subtotal thyroidectomy in 37 cases of Graves' disease, and radical thyroidectomy in 2 cases of thyroid carcinoma.

[The investigation of tranilast on the proliferation and migration of human Tenon's capsule fibroblasts].

Objective: To investigate the effect of tranilast, N-(3,4-dimethoxycinnamoyl) anthramilic acid, on the proliferation and migration of human Tenon's capsule fibroblasts.

Methods: fibroblasts were cultured from Tenon's tissue of a glaucoma patient after trabeculectomy. The subcultured cells were incubated with DMEM medium containing different concentrations of tranilast for 72 h.

Estrogen affects the differentiation and function of splenic monocyte-derived dendritic cells from normal rats.

Aim: To explore the mechanism resulting in the preventive effect of estrogen on experimental autoimmune encephalomyelitis (EAE), which is an animal model for multiple sclerosis (MS), and examine if estrogen can affect the immune response in EAE at dendritic cell (DC) level.

Methods: Flow cytometry was used to reveal the surface marker expression. 3H-thymidine incorporation was applied to examine the cellular proliferation.

Morphological observation of immunological alterations in the spleens from estrogen receptor deficient mice.

Aim: To evaluate some immunological parameters in the spleens from estrogen receptor(ER) deficient mice.

Methods: Immunohistochemistry and immunofluorescence analysis were used in the study.

Results: Multiple alterations were found in the spleens of ER deficient mice, especially in ERbeta deficient mice, such as increased expression of proinflammatory cytokines and inducible nitric oxide synthase, elevated number of proliferating splenocytes and increased IgM/IgG production.

[Morphological and quantatitive capillary changes in aging human brain].

Objective: To investigate morphological changes of capillary in aging brain and explore the role of vascular factor in brain aging.

Methods: Twenty-eight brains of individuals (mean age 65 years) who died without clinical or pathological involvement of nervous system and 6 brains of Alzheimer's disease (AD) patients (mean age 83 years) were obtained at autopsy. Sections from frontal lobe, occipital lobe, striatum and hippocampus of normal subjects and sections from hippocampus of AD patients were used for hematoxylin eosin (HE), lox fast blue (LFB), toluidine blue stains and ulex europaeus agglutinin (UEA) immunostaining.