Zebrafish as a model for Vibrio parahaemolyticus virulence.

Vibrio parahaemolyticus is a Gram-negative, naturally occurring marine bacterium. Subpopulations of strains belonging to this species cause an acute self-limiting gastroenteritis in humans and, less commonly, wound infections. In vivo models to differentiate avirulent and virulent strains and evaluate the pathogenic potential of strains of this species have been largely focused on the presence of known virulence factors such as the thermostable direct haemolysin (TDH), the TDH-related haemolysin (TRH) or the contributions of the type 3 secretion systems.

Managing crises effectively: an intervention model.

The nature of our work in health care is such that it lends itself to high levels of ambiguity at best and frequently even to the presence of crisis situations. The ability to manage crisis, therefore, is an increasingly vital skill for nurse executives. Creative and productive possibilities can arise from coping well with such serious and unusual events.

Applications of the MEGADATS database system in medical genetics.

The MEGADATS relational database system has many useful applications in the field of medical genetics. Some of these applications include storage, retrieval, and display of pedigree information; retrieval of sets of individuals, sibships, or families who meet given criteria; storage of necessary information for mailing lists, clinic data, etc; and combination of pedigree information and genotype information into the format needed for linkage analysis packages.

The use of relational database commands in retrieval of pedigree information.

Genetic research frequently requires retrieval of information about families afflicted with some genetic disease. These queries may involve simple retrieval of information on individuals with particular attributes or finding data from sibships or families who meet some set of criteria. Although the former case may be handled by almost any file management system, the latter case cannot be easily managed since family relationships are necessary to the query.

Automating data manipulation for genetic analysis using a data base management system.

Inefficient coding and manipulation of pedigree data have often hindered the progress of genetic studies. In this paper we present the methodology for interfacing a data base management system (DBMS) called MEGADATS with a linkage analysis program called LIPED. Two families that segregate a dominant trait and one test marker were used in a simulated exercise to demonstrate how a DBMS can be used to automate tedious clerical steps and improve the efficiency of a genetic analysis.

Huntington's disease research roster data base support with MEGADATS-3M.

This paper describes the MEGADATS (MEdical Genetics Acquisition and DAta Transfer System) data base development project for collecting, storing, retrieving, and plotting human family pedigrees. The newest system, MEGADATS-3M, is described. The microcomputer version of MEGADATS-3M and the use of MEGADATS-3M in the support of the Huntington's disease research roster project are emphasized.

Comparison of the metabolic and toxic effects of 2-chloropropionate and dichloroacetate.

The metabolic and toxic effects of 2-chloropropionate and dichloroacetate, activators of the pyruvate dehydrogenase complex, were compared. In 4-hr fasted mice, the oral LD50 values for 2-chloropropionate and dichloroacetate were 15.4 +/- 0.

Studies on the inhibition of gluconeogenesis by oxalate.

Oxalate was shown to enter isolated rat hepatocytes and to inhibit gluconeogenesis from lactate, pyruvate, and alanine, but not from glutamine, proline, propionate or dihydroxyacetone. Oxalate apparently acts by inhibiting pyruvate carboxylase (EC 6.4.

Development of guanylylimidodiphosphate-dependent activation of adenylate cyclase by glucagon in the neonatal rat heart.

The basal adenylate cyclase activity of the rat heart increases with the age of the animal. By itself, 10(-5) M glucagon activates only adenylate cyclase activity from adult rat hearts. In contrast, 10(-5) M glucagon in the presence of 10(-4)M 5'-guanylylimidodiphosphate (GMP-PNP) clearly activates adenylates cyclase activity in the 14-day-old rat heart, with some activation being evident in hearts of 7-day-old animals.