Quantification of protein and latex allergen content of various natural rubber latex products.

Introduction: The use of natural rubber latex (NRL) products can cause IgE-mediated allergic reactions in exposed people. The aim of this study was to quantify the content of protein and latex allergens of currently available NRL products to estimate the allergenic potential of these products.

Methods: 14 household articles (pacifiers, baby bottle nipples, condoms, household and disposable gloves, toy balloons, and Band-Aids) as well as 18 NRL examination gloves currently used by healthcare workers were investigated.

Cross-contamination of a UROtsa stock with T24 cells--molecular comparison of different cell lines and stocks.

Background: UROtsa is an authentic, immortalized human urothelial cell line that is used to study the effects of metals and other toxic substances, mostly in the context of bladder cancer carcinogenesis. Unusual properties on the molecular level of a provided UROtsa cell line stock prompted us to verify its identity.

Methods: UROtsa cell line stocks from different sources were tested on several molecular levels and compared with other cell lines.