Sensitivity-enhanced self-powered biosensing platform for detection of sugarcane smut using Mn-doped ZIF-67, RCA-DNA nano-grid array and capacitor.

Sugarcane smut is a widespread fungal disease, which severely impairs the quality and sugar yield of sugarcane. Early detection is crucial for mitigating its impact, which makes the development of a highly sensitive and accurate detection method essential. Herein, the Mn-doped zeolite imidazolate framework (ZIF-67), synthesized via a nano-confined-reactor approach, is designed to significantly enhance electron transport and boost the enzyme loading capacity within biofuel cells, thereby potentially enhancing their overall performance.

Dual-modal improved biosensing platform for sugarcane smut pathogen based on biological enzyme-Mg DNAzyme coupled with DNA transporter cascading hybridization chain reaction.

Sugarcane smut is a major disease affecting the yield and quality of sugarcane, and its early detection is crucial for the healthy development of sugarcane industry. In this work, a dual-modal biosensing platform is designed based on Au-V-MOF and 3D DNA walker for highly sensitive and precise detection of the sugarcane smut pathogen. This detection system utilizes the catalytic properties of biocatalysts and the precise cleavage of DNAzymes, along with 3D DNA walker nanotechnology and a designed "walking track", to achieve amplified detection signals and accurate target recognition.

Super signal-enhancement biosensing platform for precise target recognition based on rolling circle-hybridization chain dual linear cascade amplification technology.

This paper presents a self-powered biosensing platform based on graphdiyne@Au (2D GDY@Au) nanoparticles and rolling circle-hybridization chain (RC-HC) dual linear cascade amplification technology, which significantly enhances target recognition and signal amplification efficiency for miRNA-141. Specifically, the target on bioanode outputs a large amount of single-stranded DNA (T1) through the strand displacement amplification (SDA) mechanism. This efficient target recycling process triggers RC-HC dual linear cascade reaction.

Entropy-driven self-assembled enzyme-DNA nanomatrix cascade DNAzyme-CRISPR/cas system for multiplexed enhancement of self-powered sensing platform for protein detection.

Clustered Regularly Interspaced Short Palindromic Repeats and CRISPR-associated Proteins (CRISPR/Cas) system can accurately identify and cleave target DNA sequences, while the effective combination of DNA nanomatrix and entropy-driven self-assembled enzymes can significantly enhance the sensitivity, stability, and diversified functionality of sensors through highly ordered molecular arrangement and spontaneous efficient assembly processes. Herein, a carbon-encapsulated MoS hollow nanorod (C-MoS) with excellent conductivity and multiple active sites is used to construct bioanode of biofuel cell by integrating it with an entropy-driven self-assembled enzyme-DNA nanomatrix cascade DNAzyme-CRISPR/Cas system. When thrombin binds aptamer, it exposes the trigger strand on the anode, initiating chain displacement.

A dual-mode strategy for early detection of sugarcane pokkah boeng disease pathogen: A portable sensing device based on Cross-N DNA framework and MoS@GDY.

Sucrose, a common sugar primarily derived from sugarcane, is a crucial national strategic resource. However, its yield is significantly affected by various serious diseases, with pokkah boeng disease being one of the most damaging. Therefore, developing a sensitive method for the accurate detection of the pokkah boeng disease pathogen is crucial for ensuring the safety of sugar.