Chimeric Free Flaps Based on End-to-Side Anastomosis for Complex Lower Extremity Reconstruction.

Background: Complex lower extremity defects are difficult to cover and often require multiple free tissue transfers. Chimeric anterolateral thigh free flaps (ALTF) and peroneal artery perforator free flaps (PAPF) have been designed specifically as an alternative for reconstruction with arterial end-to-side (ETS) anastomosis. We aimed to assess our institutional experience with this technique and to define its role in complex lower extremity reconstruction.

Effects on Multimodal Connectivity Patterns in Female Schizophrenia During 8 Weeks of Antipsychotic Treatment.

Background And Hypothesis: Respective abnormal structural connectivity (SC) and functional connectivity (FC) have been reported in individuals with schizophrenia. However, transmodal associations between SC and FC following antipsychotic treatment, especially in female schizophrenia, remain unclear. We hypothesized that increased SC-FC coupling may be found in female schizophrenia, and could be normalized after antipsychotic treatment.

Fabrication of Lab-on-a-Chip Devices to Study the Forces Imparted by Growing Phytophthora Hyphae.

In this chapter, we describe the design and manufacture of a Lab-on-a-Chip (LoC) device suitable for measuring the μN forces exerted by tips of growing Phytophthora hyphae. LoC describes microfluidic devices, typically made of the polymer polydimethylsiloxane (PDMS), that are increasingly being used to answer fundamental questions in biological, chemical, physical, and medical research. These LoC devices enable the integration of several laboratory functions on small plastic devices that are quick to produce and easy to replicate.

Target protein identification in live cells and organisms with a non-diffusive proximity tagging system.

Identifying target proteins for bioactive molecules is essential for understanding their mechanisms, developing improved derivatives, and minimizing off-target effects. Despite advances in target identification (target-ID) technologies, significant challenges remain, impeding drug development. Most target-ID methods use cell lysates, but maintaining an intact cellular context is vital for capturing specific drug-protein interactions, such as those with transient protein complexes and membrane-associated proteins.

Analysis of Immunosuppression and Antioxidant Damage in Diploid and Triploid Crucian Carp () Induced by Saline-Alkaline Environmental Stress: From Metabolomic Insight.

The salinization of the water environment worldwide is increasing, which has brought great challenges to the sustainability of fish farming of aquatic animals. Three NaHCO concentration groups (0 mmol/L, 20 mmol/L, and 60 mmol/L) were set up in this study to investigate growth and metabolic differences between diploid and triploid crucian carp under saline-alkaline stresses. This study utilized UPLC-QTOF/MS metabolomics to analyze significant metabolites and metabolic pathways in the serum of diploid and triploid crucian carp, exposing them to different NaHCO concentrations in saline-alkaline habitats, elucidating the mechanism of their metabolic differences.