Navigating Limited Resources-Outpatient Pediatric Cholecystectomies at Rural Hospitals.

Background: Rural facilities that provide pediatric surgical services are a critical resource to local communities. Our aim was to characterize differences in outpatient pediatric cholecystectomy outcomes performed at rural and urban hospitals with the hypothesis that rural hospitals would have similar outcomes.

Methods: The Nationwide Ambulatory Surgery Sample (NASS), which contains ambulatory surgery encounters at hospital-owned facilities, was used to perform a retrospective cohort analysis of pediatric patients age 18-years and younger who had a cholecystectomy (n = 15,449) between 2016 and 2018.

Genomic Insights into TFRC-KFRI-1 Isolated from Manila Clam (): Functional and Phylogenetic Analysis.

TFRC-KFRI-1, isolated from the gut of Manila Clam in the sediment of the West Sea of Korea, was investigated for its potential as a probiotic bacterium. This strain, belonging to the family , was previously classified as but later reclassified to the genus , known for species with bioremediation and probiotic properties. To evaluate its genomic features and potential applications, we performed draft-genome sequencing and analysis.

Constructing Three-Dimensional Porous SnS/RGO as Superior-Rate and Long-Life Anodes for Lithium-Ion Batteries.

Tin-based sulfides, possessing a unique layered structure and a high theoretical capacity, stand as highly prospective contenders for anode materials in lithium-ion batteries (LIBs). Nevertheless, the pronounced volume expansion that occurs during lithium storage and poor capacity retention have limited its progress toward commercialization. Herein, we designed and prepared a SnS/RGO composite with a three-dimensional porous structure by sulfurizing the SnO(OH)/GO precursor.

Free Energy-Based Refinement of DNA Force Field via Modification of Multiple Nonbonding Energy Terms.

The amber-OL21 force field (ff) was developed to better describe noncanonical DNA, including Z-DNA. Despite its improvements for DNA simulations, this study found that OL21's scope of application was limited by embedded ff artifacts. In a benchmark set of seven DNA molecules, including two double-stranded DNAs transitioning between B- and Z-DNA and five single-stranded DNAs folding into mini-dumbbell or G-quadruplex structures, the free energy landscapes obtained using OL21 revealed several issues: Z-DNA was overly stabilized; misfolded states in mini-dumbbell DNAs were most stable; DNA GQ folding was consistently biased toward an antiparallel topology.

Free Energy Landscape of Lesion Recognition by Human 8-Oxoguanine DNA N-Glycosylase 1: Mechanistic Insights into Detection and Processing of 8-Oxoguanine in DNA.

Human 8-oxoguanine DNA -glycosylase 1 (hOGG1) is an essential enzyme in DNA repair, responsible for recognizing and excising 8-oxoguanine (8OG), the lesion resulting from oxidative damage to guanine (G). By removing 8OG, hOGG1 prevents mutations like G-to-T transversions, maintains genomic stability, and reduces the risk of cancer and other diseases. Structural studies of hOGG1 bound to DNA have shown that lesion recognition occurs through base eversion from the DNA helix and hOGG1 finger residue insertion into the DNA helix.