Publications by authors named "Y V Gervaziev"
Article Synopsis
- Stellate genes, located on the X chromosome of Drosophila melanogaster, are essential for male fertility and encode a protein similar to a subunit of protein kinase CK2.
- Over-expression of these genes due to derepression leads to meiotic disruptions and male sterility.
- The study identifies important E-boxes in the Stellate gene promoters that interact with the dUSF protein, highlighting their role in regulating gene expression specifically in the testes.
IL-4 is a pleiotropic immunoregulatory cytokine secreted by Th2 subset of CD4(+) Th cells. Several transcription factors (TFs) have been determined with various degrees of certainty to bind the IL-4 promoter and to regulate its expression in human. To investigate the mechanisms responsible for phenotypic effects of the C-33T IL-4 promoter polymorphism, we performed a search of TFs binding to this promoter locus and discriminating the -33C and -33T alleles.
View Article and Find Full Text PDFBackground: Susceptibility to the development of bronchial asthma (BA) and other atopic diseases is known to be associated with genetic components.
Objective: To evaluate the possible role of the polymorphisms in IL-4 gene promoters (C-33T, C-590T and G-1098T) in modulating allergic response and asthma in the Russian population.
Methods: The polymorphism analysis was carried out by PCR-RFLP; IL-4 and total IgE concentrations were determined by ELISA.
ECAR-LANS, the recombinant L-asparaginase from Erwinia carotovora, is a prospective therapeutic enzyme for leukaemia treatment. An efficient and economical scheme was developed for the purification, cloning and expression in Eschericha coli of ECAR-LANS. More than 90% purity, complemented with 72% active enzyme recovery, was achieved with a single chromatographic purification step.
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