Publications by authors named "Y Tomabechi"
We investigated the transglycosylation reaction of two types of oligosaccharide acceptors, i.e., β-cyclodextrin (CD) derivatives 1 and 2 conjugated with multiple glucose (Glc) units, catalyzed by endo-β-N-acetyl-glucosaminidase from Mucor hiemalis (Endo-M) using the oligosaccharide donor sialoglycopeptide (SGP).
View Article and Find Full Text PDFProtein phosphatase 2A (PP2A) is an essential serine/threonine protein phosphatase, and its dysfunction is involved in the onset of cancer and neurodegenerative disorders. PP2A functions as a trimeric holoenzyme whose composition is regulated by the methyl-esterification (methylation) of the PP2A catalytic subunit (PP2Ac). Protein phosphatase methylesterase-1 (PME-1) is the sole PP2Ac methylesterase, and the higher PME-1 expression is observed in various cancer and neurodegenerative diseases.
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- - Several antibody therapeutics exist for SARS-CoV-2, but their effectiveness has decreased against emerging variants, prompting researchers to explore new antibodies obtained from recovered COVID-19 patients' B cells.
- - From 172 antibodies created using the Wuhan strain and Gamma variant, six were effective against earlier strains, while five showed some ability to combat Omicron sub-strains, indicating a potential for broader neutralization.
- - Testing one promising antibody in hamsters showed a significant reduction in lung viral levels, demonstrating its potential as an antiviral treatment and underscoring the need for effective initial screening in developing such therapies.
Article Synopsis
- Researchers developed 494 monoclonal antibodies from COVID-19 convalescent patients and found some that effectively neutralize SARS-CoV-2, including its variants, similar to existing clinical antibodies.
- The antibodies demonstrated varied effectiveness against different virus mutations and were validated through cell-based assays and cryo-electron microscopy.
- Therapeutic tests in hamster and macaque models showed that these antibodies, especially in a cocktail form, significantly reduced viral levels and lung damage, indicating their potential as therapeutic options against COVID-19.
Genetically encoded caged amino acids can be used to control the dynamics of protein activities and cellular localization in response to external cues. In the present study, we revealed the structural basis for the recognition of -(2-nitrobenzyl)-L-tyrosine (NBTyr) by its specific variant of tyrosyl-tRNA synthetase (NBTyrRS), and then demonstrated its potential availability for time-resolved X-ray crystallography. The substrate-bound crystal structure of NBTyrRS at a 2.
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