A simplified microwell pseudoreplica for the detection of viruses by electron microscopy and immunoelectron microscopy.

Simplified procedures for immunoelectron microscopy (IEM) and electron microscopy (EM) are described. The procedures employ the principle of agar filtration and pseudoreplication. The modification consisted of the use of microwells for storage of gels with or without antiserum (for IEM or EM, respectively) and an array of containers in which pseudoreplication and negative staining were performed.

Evaluation of immunoadsorbent electron microscopic techniques for detection of Sindbis virus.

Two immunosorbent electron microscopic techniques (ISEM), the protein A coated grid technique (PA-CGT) and the antibody coated grid technique (AB-CGT) were applied and evaluated for the detection of Sindbis virus from infected tissue culture fluids. At optimal conditions, the efficiency of trapping the virions was only about 1.5 higher with the PA-CGT as compared to the AB-CGT, but the PA-CGT was less dependent on the antiserum dilution used in the test.

Differentiation and myelination of transplanted mouse dorsal root ganglia.

Dorsal root ganglia from newborn mice develop and myelinate following transplantation under the kidney capsule of adult syngeneic mice. The transplanted dorsal root ganglia in the subcapsular environment developed more rapidly and formed more abundant myelin than comparable ganglia in an in vitro culture.

Silicates as nonspecific adsorbents of bacteriophage: a model for purification of water from viruses.

Amorphous silicates having small particles with a large surface area were found to have high nonspecific adsorption capacity. Investigations with Escherichia coli T4 bacteriophage have shown adsorption capacity of up to 99.9% on different tested silicates.

Solid-phase immune electron microscopy (SPIEM) for rapid viral diagnosis.

Immune electron microscopy (IEM) is one of the fastest and most sensitive methods for the detection and diagnosis of viruses. This technique is based on formation of immune complexes of the virus with its corresponding antibody. In IEM optimal precipitation depends on a correct ratio, and there is a prozone effect.