Publications by authors named "Y Seita"
Article Synopsis
- Aberrant male germline development can cause a type of cancer called seminoma, which is a tumor in the testicles.
- Seminomas are similar to early-stage germ cells and often have an extra piece of chromosome 12.
- Researchers are studying seminomas using special cells from patients to understand what causes this cancer and how it forms, since there aren't any mouse models to help with this research.
Despite our increasing knowledge of molecular mechanisms guiding various aspects of human reproduction, those underlying human primordial germ cell (PGC) development remain largely unknown. Here, we conducted custom CRISPR screening in an in vitro system of human PGC-like cells (hPGCLCs) to identify genes required for acquisition and maintenance of PGC fate. Amongst our candidates, we identified TCL1A, an AKT coactivator.
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- Familial hypercholesterolemia (FH) is a genetic disorder that leads to high LDL cholesterol levels and increased cardiovascular disease risk.
- Researchers created a model of FH in cynomolgus monkeys by using genome editing to knock out the LDL receptor gene, resulting in six confirmed LDLR knockout monkeys.
- These monkeys exhibited high plasma cholesterol and triglyceride levels similar to FH patients, along with observed symptoms like xanthomas, and showed resistance to common hypercholesterolemia treatments.
There is a scarcity of information regarding the molecular mechanisms underlying human germ cell development due to limitations in obtaining the relevant materials. Reconstitution of human germ cell development from pluripotent stem cells in vitro would provide critical insight into the etiology of various reproductive conditions and disorders, including infertility.Recently, we reported the in vitro reconstitution of human prospermatogonial development from human-induced pluripotent stem cells through human primordial germ cell (PGC)-like cells (hPGCLCs) using long-term cultured xenogeneic reconstituted testes.
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- This study explores how to transform pluripotent stem cells into germ cells, specifically in the common marmoset, enhancing our understanding of germ cell development.
- Researchers established methods to culture induced pluripotent stem cells (iPSCs) that can differentiate into primordial germ cell-like cells (PGCLCs), closely resembling the marmoset's natural germ cells.
- The findings provide valuable insights for future research on human gametogenesis, potentially aiding in preclinical modeling of reproductive development.