A comparative study of the effects of three root-end filling materials on proliferation and adherence of human periodontal ligament fibroblasts.

Introduction: The present in vitro study was conducted with the aim of evaluating and comparing the cytotoxic effects of three root-end filling materials, ProRoot mineral trioxide aggregate (ProRoot MTA; Dentsply Tulsa Dental, Memphis, TN), MTA Angelus (Angelus, Londrina, Brazil), and a modified zinc oxide-eugenol cement (Super-EBA; Bosworth Co, Skokie, IL) on human periodontal ligament (PDL) fibroblasts.

Methods: PDL cells were cultured in an mineral trioxide aggregate (MTA)- or a Super-EBA-conditioned medium to assess the viability as determined by the trypan blue exclusion assay. The proliferation of the cells was recorded, and the cellular morphology was observed by confocal microscopy.

Beta-cell function abnormalities in islets from an adult subject with nesidioblastosis and autoantibodies against the islet cells.

Human islets from an adult subject with nesidioblastosis were isolated and used to perform in vitro studies. Isolated nesidioblastotic islets showed an increased basal rate of insulin secretion (nesidioblastotic islets, 81.3 +/- 6.

Functional properties of isolated human pancreatic islets beneficial effects of culture and exposure to high glucose concentrations.

In order to assess the functional properties of human pancreatic islets we have evaluated insulin secretion, glucose metabolism and insulin mRNA synthesis. Particular attention was given to evaluate how culture and exposure to high glucose concentrations "in-vitro" influence the subsequent function of these cells. Islets were cultured for 1 and 7 days at 5.

Deleterious effect of dithizone-DMSO staining on insulin secretion in rat and human pancreatic islets.

Dithizone (DTZ) is a selective stain for pancreatic islets which facilitates their identification, being of special interest in human islet isolation assessment. Nevertheless, there are few studies concerning its potential toxic effects on islet function. In our study, we have evaluated the effects of DTZ (dissolved in dimethyl sulfoxide [DMSO] 1% w/v) at three different concentrations (2, 10, and 100 micrograms/ml) on insulin response to glucose in human and rat islets.

Regulation of islet amyloid polypeptide in human pancreatic islets.

This study investigated the effect of glucose on islet amyloid polypeptide secretion, content, and mRNA synthesis of human pancreatic islets. The release of islet amyloid polypeptide from fresh isolated islets in response to glucose was parallel to that of insulin. The islet amyloid polypeptide-to-insulin molar ratios in response to 5.