Characterization of Klebsiella pneumoniae Carbapenemase (KPC)-14, a KPC Variant Conferring Resistance to Ceftazidime-Avibactam in the Extensively Drug-resistant ST463 Pseudomonas aeruginosa Clinical Isolate.

Objectives: We studied two Klebsiella pneumoniae carbapenemase (KPC)-14 variants from clinical Pseudomonas aeruginosa isolates (C137 and C159) to better understand the genomic diversity, mechanisms, and genes that confer antibiotic resistance and pathogenicity.

Methods: Genomic DNA from C137/159 was subjected to Illumina and Oxford Nanopore sequencing. Horizontal transmission of the plasmid was evaluated using cloning experiments.

TRIM8 enhances chondrocyte ferroptosis by inhibiting YTHDF2-m6A mediated SREBF2 mRNA degradation to promote OA progression.

Chondrocyte ferroptosis plays a crucial role in osteoarthritis (OA) progression. Our previous study demonstrated that TRIM8 knockdown alleviated IL-1β-induced chondrocyte injury. However, the involvement of TRIM8 in regulating OA progression through ferroptosis remains unclear.

Effects of chili meal supplementation on productive performance, intestinal health, and liver lipid metabolism of laying hens fed low-protein diets.

This study aimed to explore the effects of chili meal (CM), a by-product of chili pepper oil extraction, on the productive performance, intestinal health, and lipid metabolism of laying hens fed low-protein (LP) diets. A total of 384 Hy-Line brown laying hens (32 weeks old) were divided into six groups: control (CON) diet with 16.5 % crude protein (CP), LP diet with 15 % CP, and LP diets supplemented with 3 %, 5 %, 7 %, and 9 % CM.

Effects of exogenous mineral silicon on physiological characteristics and arsenic uptake and transport in rice.

Soil arsenic (As) contamination poses a significant threat to food security and human health. Diatomaceous earth (DE) as a representative exogenous mineral silicon can effectively regulate the migration and transformation of As in the soil-rice system. However, the mechanisms underlying the effects of DE application on As uptake, translocation, and stress regulation in rice remain unclear.

Effects of Pichia sp. J1 and Plant Growth-Promoting Bacterium on Enhancing Tobacco Growth and Suppressing Bacterial Wilt.

Tobacco bacterial wilt (TBW), caused by Ralstonia solanacearum, significantly impacts tobacco yield and quality, leading to substantial economic losses. This study investigated the effects of the microbial agents JX (Pichia sp. J1 and Klebsiella oxytoca ZS4) on the soil properties, rhizospheric microbial community, tobacco agronomic traits, and TBW incidence through field experiments.