Target Bioconjugation of Protein Through Chemical, Molecular Dynamics, and Artificial Intelligence Approaches.

Covalent modification of proteins at specific, predetermined sites is essential for advancing biological and biopharmaceutical applications. Site-selective labeling techniques for protein modification allow us to effectively track biological function, intracellular dynamics, and localization. Despite numerous reports on modifying target proteins with functional chemical probes, unique organic reactions that achieve site-selective integration without compromising native functional properties remain a significant challenge.

Prognostic Implication of Computational Angiography-Derived Fractional Flow Reserve in Patients With Nonobstructive Coronary Artery Disease.

Background: Risk stratification of patients with symptomatic nonobstructive coronary artery disease remains uncertain. Our study assessed the clinical value of single-vessel, multivessel, and 3-vessel computational angiography-derived fractional flow reserve (caFFR) measurement in patients with nonobstructive coronary artery disease.

Methods And Results: We enrolled patients with ≤50% stenosis with a caFFR value ≥0.

Generation of Isolated Flat Bands with Tunable Numbers through Moiré Engineering.

In contrast to the Dirac-cone materials in which the low-energy spectrum features a pseudospin-1/2 structure, Lieb and Dice lattices both host triply degenerate low-energy excitations. Here, we discuss moiré structures involving twisted bilayers of these lattices, which are shown to exhibit a tunable number of isolated flat bands near the Fermi level due to the bipartite nature of their structures. These flat bands remain isolated from the high-energy bands even in the presence of small higher-order terms and chiral-symmetry-breaking interlayer tunneling.

Advancing precision therapy for colorectal cancer: Developing clinical indications for multi-target kinase inhibitor BPR1J481 using patient-derived xenograft models.

The large and rapid increase in the incidence and mortality of colorectal cancer (CRC) demonstrates the urgent need for new drugs with higher efficacy to treat CRC. However, the lack of applicable and reliable preclinical models significantly hinders the progress of drug development. Patient-derived xenograft (PDX) models are currently considered reliable in vivo preclinical models for predicting drug efficacy in cancer patients.

Protogenin facilitates trunk-to-tail HOX code transition via modulating GDF11/SMAD2 signaling in mammalian embryos.

During embryogenesis, vertebral axial patterning is intricately regulated by multiple signaling networks. This study elucidates the role of protogenin (Prtg), an immunoglobulin superfamily member, in vertebral patterning control. Prtg knockout (Prtg) mice manifest anterior homeotic transformations in their vertebral columns and significant alterations in homeobox (Hox) gene expression.