[Contribution of high pressure to pharmaceutical and medical science].

Since the beginning of the 20th century, effects of high pressure on biological systems have been studied, but the first applications in this domain have been developed in the 90's and concerned the preservation of food-stuff. Hence, much research work has been undertaken in order to develop high pressure effects in Biosciences. In the last decade, new methods or processes using high pressure (obtaining therapeutic molecules; decontamination or sterilization of biological stuff, sensitive drugs and drug carriers; development of vaccines; using high pressure as a tool in order to simulate and explore the mechanisms of proteins aggregation) underlining the potentialities of this technology in Medical and Pharmaceutical Sciences.

Effects of high hydrostatic pressure on several sensitive therapeutic molecules and a soft nanodispersed drug delivery system.

Purpose: According to the development in the last decade of industrial processes using high hydrostatic pressure (HHP) for preservation of several commercial food products, novel sterilization or decontamination processes for pharmaceutical products could be conceivable. The aim of this work is to evaluate the effects of HHP on the integrity of insulin and heparin solutions, suspension of monoclonal antibodies and Spherulites.

Methods: High performance liquid chromatography, thin layer chromatography, capillary electrophoresis assays, ELISA tests, laser granulometry and spectrophotometry analyses have been performed to compare HHP treated drugs (in a domain of pressure and temperature ranging respectively from 20 up to 500 MPa and from 20 degrees C up to 37 degrees C) vs.

The stenlying effect of high hydrostatic pressure on thermally and hydrolytically labile nanosized carriers.

Purpose: To investigate whether high hydrostatic pressure (HHP) treatment allows the sterilization of thermosensitive polymer nanoparticle suspensions without jeopardizing their physicochemical integrity.

Methods: Application of HHP was explored on a wide variety of thermosensitive poly(cyanoacrylate) nanoparticles, varying by their type (nanospheres or nanocapsules), by their preparation method (nanoprecipitation or emulsion/solvent evaporation), as well as by their surface characteristics. Physicochemical characterization before and after pressurization included turbidimetry, size measurement, zeta potential, scanning electron microscopy and infrared analysis.