Establishment of Recombinant Gene Rabies Virus SRV and Identification of Its Biological Characteristics.

Canids act as a crucial intermediary in the transmission of rabies and , serving as co-infection hosts and pathogen carriers for both rabies and hydatid disease (HD) transmitted from animals to humans. Therefore, an effective and efficient bivalent oral vaccine for preventing HD and rabies is urgently required to reduce economic losses in husbandry resulting from rabies and HD. In this study, a full-length plasmid (pcDNA4-NPM+G+EgM123+eGFP+L) carrying the gene and fluorescence reporter genes of eGFP and four auxiliary transfection plasmids of rabies virus SRV (pcDNA4-N, pcDNA4-P, pcDNA4-G, pcDNA-L) were established by reverse genetics approaches and co-transfected to BSR cells by electrotransfection.

MaxEnt-Based Predictions of Suitable Potential Distribution of Under Current and Future Climate Change.

Grassland degradation is a serious ecological issue in the farming-pastoral ecotone of northern China. Utilizing native grasses for the restoration of degraded grasslands is an effective technological approach. is a superior indigenous grass species for grassland ecological restoration in northern China.

The Improvement Effects of BC99 on Liver Function and Gut Microbiota of Long-Term Alcohol Drinkers: A Randomized Double-Blind Clinical Trial.

Background/objectives: With the improvement of living standards, alcoholic liver disease caused by long-term drinking has been a common multiple disease. Probiotic interventions may help mitigate liver damage caused by alcohol intake, but the mechanisms need more investigation.

Methods: This study involved 70 long-term alcohol drinkers (18-65 years old, alcohol consumption ≥20 g/day, lasting for more than one year) who were randomly assigned to either the BC99 group or the placebo group.

Estimation of 1 km Dawn-Dusk All-Sky Land Surface Temperature Using a Random Forest-Based Reanalysis and Thermal Infrared Remote Sensing Data Merging (RFRTM) Method.

All-sky 1 km land surface temperature (LST) data are urgently needed. Two widely applied approaches to derive such LST data are merging thermal infrared remote sensing (TIR)-passive microwave remote sensing (PMW) observations and merging TIR reanalysis data. However, as only the Moderate Resolution Imaging Spectroradiometer (MODIS) is adopted as the TIR source for merging, current 1 km all-sky LST products are limited to the MODIS observation time.

Range Extension of Borehole Strainmeters Using MOSFET-Based Multi-Switch Automatic Zero Setting.

Borehole strainmeters are essential tools for observing crustal deformation. In long-term observational applications, the dynamic changes in crustal deformation over multi-year scales often exceed the single measurement range of borehole strainmeters. Expanding the measurement range while maintaining high precision is a critical technical challenge.