Publications by authors named "Y Petryk Petryk"
Article Synopsis
- * Researchers used single-domain nanobodies from camels to target a specific enzyme (β-1,3-glucanosyltransferases) crucial for fungal survival, revealing important structural insights.
- * The tested nanobody showed strong antifungal effects in laboratory settings and in animal models, particularly against certain strains of C. neoformans, suggesting it could be a promising avenue for developing new antifungal therapies.
Conditions altering the yeast cell wall lead to the activation of an adaptive transcriptional response mainly governed by the cell wall integrity (CWI) mitogen-activated protein kinase (MAPK) pathway. Two high-throughput screenings were developed using the yTHC collection of yeast conditional mutant strains to systematically identify essential genes related to cell wall integrity, and those required for the transcriptional program elicited by cell wall stress. Depleted expression of 52 essential genes resulted in hypersensitivity to the dye Calcofluor white, with chromatin organization, Golgi vesicle transport, rRNA processing, and protein glycosylation processes, as the most highly representative functional groups.
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