Growth-stimulating effect of pharmacological doses of glucocorticoid on androgen-responsive Shionogi carcinoma 115 in vivo in mice and in cell culture.

It has been generally accepted for 20 years that the growth of Shionogi carcinoma 115 (SC115) is stimulated only by androgen. However, we recently found that the growth of SC115 cells is also stimulated by pharmacological doses of estrogen in vivo but not in cell culture. In the present study, the growth-stimulatory effect of glucocorticoid on SC115 cells was examined.

Difference in extractability of estradiol- and tamoxifen-receptor complex in the nuclei from MCF-7 cells with Nonidet P-40.

The extraction of [3H]estradiol- and [3H]tamoxifen-receptor complex in the nuclei from MCF-7 cells with the nonionic detergent Nonidet P-40 has been studied. We found that there is a striking difference in the extractability of estradiol- and tamoxifen-receptor complex from nuclei with 0.5% Nonidet P-40.

Effects of thiocyanate on cytosol androgen receptor from Shionogi carcinoma 115.

The effects of KSCN and other chaotropic salts on the androgen receptor in cytosol of Shionogi carcinoma 115 were studied by means of charcoal adsorption assay and sucrose gradient centrifugation. When KSCN or NaSCN was added to the [3H]-dihydrotestosterone-cytosol mixture at the final concentration of 0.5 M, the androgen binding to the cytosol receptor was considerably inhibited.

Apparent ineffectiveness of adriamycin for growth of androgen-dependent Shionogi carcinoma 115 in the mouse.

Although adriamycin (1 mg/kg) and 5-fluorouracil (25 mg/kg) were equally effective in inhibiting the growth of Ehrlich carcinoma, both ascites and solid forms, in the mouse, the growth of Shionogi carcinoma 115 in the male DS mouse was relatively resistant to the treatment with adriamycin as compared to 5-fluorouracil. However, direct cytotoxic activity of adriamycin in cultured Shionogi carcinoma 115 (SC 115) cells was about 1000-fold molar potent when compared with 5-fluorouracil. The apparent ineffectiveness of adriamycin against SC 115 in the DS mouse seems to be, at least in part, due to the depression of host defence mechanisms.

Stimulatory effects of dexamethasone and indomethacin on growth of androgen-dependent Shionogi carcinoma 115 in the mouse.

The effects of dexamethasone and indomethacin on the growth of androgen-dependent Shionogi carcinoma 115 were studied. When castrated male DS mice were treated with dexamethasone for 2 weeks at daily doses of 1-5 mg/kg starting from 3 days before tumor inoculation, the tumor weight as well as the transplantability was significantly increased. The tumor grown in dexamethasone-treated mice had morphological, biochemical and biological characteristics similar to those of the original Shionogi carcinoma 115.