Establishment and characterization of a renal cell carcinoma cell line (FU-UR-1) with the reciprocal ASPL-TFE3 fusion transcript.

We have established a cultured cell line named FU-UR-1 from a large retroperitoneal tumor of a 24-year-old Japanese male patient who simultaneously had a small renal cell carcinoma (RCC). Cytogenetic analysis and fluorescence in situ hybridization of the retroperitoneal tumor, and the cell line established from this tumor demonstrated similar karyotypes including add(13)(p11).ish der(13)t(13;17) (p11;q11)t(X;17)(p11;q25)(wcpX+,wcp17+).

Chromosomal imbalances in angioleiomyomas by comparative genomic hybridization.

Angioleiomyoma is a benign soft tissue tumor that usually develops in the subcutis of the lower extremities. It characteristically consists of thick vessel walls formed by proliferating smooth muscle cells, and vascular channels. Very little is known about the molecular cytogenetic changes in angioleiomyoma.

Establishment of a new human malignant fibrous histiocytoma cell line, FU-MFH-1: cytogenetic characterization by comparative genomic hybridization and fluorescence in situ hybridization.

Although a number of malignant fibrous histiocytoma (MFH) cell lines have been reported, their characterization at a molecular cytogenetic level has not been fully established. In this study, we established a new human cell line, designated as FU-MFH-1, from a storiform-pleomorphic MFH arising in the retroperitoneum of a 61-year-old woman, and applied comparative genomic hybridization (CGH) and fluorescence in situ hybridization (FISH) with chromosome painting probes for the characterization of chromosome alterations. FU-MFH-1 cells were spindle, round, or polygonal in shape with oval nuclei, and were maintained continuously in vitro for over 50 passages for more than 12 months.