Publications by authors named "Y NOMIYA"
We assessed the feasibility of transplanting a sheet of retinal pigment epithelial (RPE) cells differentiated from induced pluripotent stem cells (iPSCs) in a patient with neovascular age-related macular degeneration. The iPSCs were generated from skin fibroblasts obtained from two patients with advanced neovascular age-related macular degeneration and were differentiated into RPE cells. The RPE cells and the iPSCs from which they were derived were subject to extensive testing.
View Article and Find Full Text PDFBackground: Approximately 10-20% of Kawasaki disease (KD) patients are resistant to intravenous immunoglobulin (IVIG) treatment. Further, these patients are at a particularly high risk of having coronary artery abnormalities. The mechanisms of IVIG resistance in KD have been analyzed using patient leukocytes, but not patient vascular endothelial cells (ECs).
View Article and Find Full Text PDFCapZ (β-actinin) and tropomodulin (Tmod) are capping proteins involved in the maintenance of thin filaments in vertebrate skeletal muscles. In this study, we focused on amphioxus, the most primitive chordate. We searched for CapZ and Tmod genes in the amphioxus genome and determined their primary structures.
View Article and Find Full Text PDFDetermining the complete primary structure of large proteins is difficult because of the large sequence size and low sequence homology among animals, as is the case with connectin (titin)-like proteins in invertebrate muscles. Conventionally, large proteins have been investigated using immuno-screenings and plaque hybridization screenings that require significant time and labor. Recently, however, the genomic sequences of various invertebrates have been determined, leading to changes in the strategies used to elucidate the complete primary structures of large proteins.
View Article and Find Full Text PDFArticle Synopsis
- The study introduces a directed evolution strategy that focuses on fine-tuning multiple mutations to enhance specific properties of enzymes while minimizing negative impacts on other functions.
- Researchers used lactate oxidase (LOX) as a model, revealing a trade-off between its thermostability and catalytic activity through mutation profiling.
- By selecting and combining mutations that increased catalytic activity without significantly affecting thermostability, the resulting enzyme mutants showed significant performance improvements compared to those developed with generic thermostable mutations.