Evaluation of Cytotoxicity of the Dental Materials TheraCal LC, TheraCal PT, ApaCal ART and Biodentine Used in Vital Pulp Therapy: In Vitro Study.

(1) Background: The aim of this study was to compare the cytotoxicity of selected resin-modified materials used in direct contact with the dental pulp (TheraCal LC, TheraCal PT, and ApaCal ART) with calcium silicate cement (Biodentine). (2) Methods: The mouse fibroblast Balb/3T3 cell line and the extracts of tested materials in four concentrations were used for the testing. An MTT assay was performed in three independent experiments with six replicates for each concentration of tested material.

The Use of Umbilical Cord Blood Nucleated Cells in the Treatment of Regressive Autism: A Case Report.

Background: Interest in the issue of childhood autism has surged in the recent decades. At the same time, despite the significant progress achieved in understanding the etiological and pathogenetic aspects of the condition, effective ways to treat it have continued to elude us. Stem cell therapy appears to hold great promise in the treatment and rehabilitation of patients with both neurological diseases (cerebral palsy, hydrocephalus) and mental disorders (autism, schizophrenia).

Characterization of the Biosynthetic Gene Cluster for the Ribosomally Synthesized Cyclic Peptide Epichloëcyclins in .

The various grass-induced epichloëcyclins of the spp. are ribosomally synthesized and post-translationally modified peptides (RiPPs), produced as small, secreted cyclopeptides from a single gene, Here, four clustered and coregulated genes (, , , and ) with predicted roles in epichloëcyclin production in were evaluated through gene disruption. Subsequent chemical analysis indicates that GigB is a DUF3328 domain-containing protein associated with cyclization of epichloëcyclins; GigC is a methyltransferase enzyme responsible for N-methylation of desmethylepichloëcyclins; and KexB is a subtilisin-like enzyme, partly responsible for the propeptide cleavage of epichloëcyclin intermediates.

Survey of the Endophytic Bacteria Inhabiting Wild Seed Using 16S rRNA Gene Amplicon Sequencing.

We report a preliminary survey of the endophytic bacterial microbiota of seed from wild carrot (Daucus carota) using 16S rRNA gene amplicon sequencing. , , , and were the most abundant phyla detected, while , , , , Pseudomonas, , , and were the most abundant genera.

Evaluation of the Feasibility of a Simultaneous Change in the Fractional Composition and the Involvement of Depressant Additives to Obtain Low-Freezing Diesel Fuels.

In this paper, the characteristics of a straight-run diesel fuel sample, narrow diesel fractions, and their blends with a depressant additive are tested and analyzed. The efficiency of the depressant additive action on the low-temperature properties of narrow diesel fractions is evaluated. It is shown that the depressant additive is most effective on the pour point of the heavy diesel fraction (300-360 °C) and cold filter plugging point of the light diesel fraction (180-240 °C).