Publications by authors named "Y Motorin"
Article Synopsis
- Tgt enzyme modifies guanine in tRNAs with GUN anticodon to queuosine, which is crucial for bacterial growth under aminoglycoside stress.
- Research highlights the significance of Q34 modification in enhancing decoding efficiency of specific codons (TAT and TAC) during tobramycin exposure.
- Findings suggest that Q34 regulation can lead to translational reprogramming impacting genes like RsxA, crucial for the bacterial response to oxidative stress and antibiotics.
Article Synopsis
- Intrinsically disordered regions (IDRs) are abundant in nucleolar proteins but their specific role in ribosome assembly is not well understood.
- The study demonstrates that lysine-rich IDRs in small nucleolar ribonucleoprotein particles (snoRNPs) aid in the modification of precursor rRNA and play a crucial role in nucleolar organization during stress.
- The findings suggest that these IDRs might serve as an evolutionary regulatory mechanism, allowing eukaryotic cells to adapt nucleolar structure based on rRNA production needs.
Article Synopsis
- The study investigates how dihydrouridine modifications in tRNA affect gene expression during stress, particularly in response to oxidative conditions in Escherichia coli.
- Researchers manipulated the redox state using paraquat and found that different tRNA-dihydrouridine synthases (DusA, DusB, DusC) have varying sensitivities to oxidative stress, influencing dihydrouridine levels.
- The findings highlight the role of NADPH availability in tRNA modification and provide new insights into how cells respond to oxidative stress through redox dynamics.
Dysregulation of DNA methylation is associated with human disease, particularly cancer, and the assessment of aberrant methylation patterns holds great promise for clinical diagnostics. However, DNA polymerases do not effectively discriminate between processing 5-methylcytosine (5 mC) and unmethylated cytosine, resulting in the silencing of methylation information during amplification or sequencing. As a result, current detection methods require multi-step DNA conversion treatments or careful analysis of sequencing data to decipher individual 5 mC bases.
View Article and Find Full Text PDFRNA modifications play a pivotal role in the regulation of RNA chemistry within cells. Several technologies have been developed with the goal of using RNA modifications to regulate cellular biochemistry selectively, but achieving selective and precise modifications remains a challenge. Here, we show that by using designer organelles, we can modify mRNA with pseudouridine in a highly selective and guide-RNA-dependent manner.
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