Characterization of quorum quenching enzyme AiiA and its potential role in strawberry preservation.

Microbial spoilage in nutrient-rich strawberries has led to significant food waste and economic loss in the food industry. The quorum-quenching enzyme AiiA is believed to disrupt communication between cells by inactivating AHLs-based quorum sensing signals. Chitosan, a biopolymer derived from chitin, is widely used as a coating film to inhibit bacterial growth and prolong food shelf life.

Chelation treatment for heavy metals in municipal solid waste incineration fly ash: 300-Day study on stability and environmental risk.

Landfilling after chelation is the primary method for treating municipal solid waste incineration fly ash (MSWI FA), but the long-term environmental risks of its heavy metals (HMs) remain unclear. This study used three common organic chelating agents (CAs) to stabilize MSWI FA and assessed its 300-day stability under mixed and zoned landfill scenarios. The findings showed that Pb leaching posed high environmental risks, especially in zoned landfills, with 10-year cumulative leaching of 300 mg/kg, while Cu and Zn leaching remained within limits, indicating lower environmental risks.

Gengnianchun formula ameliorates insulin resistance-induced diminished ovarian reserve via the estrogen signaling pathway: evidence from network pharmacology and experimental validation.

Background: Diminished ovarian reserve (DOR), a major cause of female infertility, is closely linked to insulin resistance (IR). Traditional Chinese Medicine (TCM) approaches, such as the Gengnianchun (GNC) formula, focus on restoring ovarian function by improving IR and regulating hormonal balance. Despite GNC's demonstrated efficacy, its precise therapeutic mechanisms remain unclear.

Protein engineering, expression optimization, and application of alkaline protease from Alkalihalobacillus clausii FYX.

Alkaline protease has been commercially used in the areas of detergents, food and agriculture, and improving the performance and production of alkaline protease serves as an important role in promoting its market expansion. Here, an alkaline protease AE0 from Alkalihalobacillus clausii FYX was firstly characterized in Bacillus licheniformis DW2△aprE, the optimal temperature and pH of AE0 were 60 °C and 10.5, the K and K values for casein were 17.

Electroacupuncture produces analgesic effects via cannabinoid CB1 receptor-mediated GABAergic neuronal inhibition in the rostral ventromedial medulla.

Objective: Electroacupuncture (EA) is commonly used for pain control in clinical practice, yet the precise mechanisms underlying its action are not fully understood. The rostral ventromedial medulla (RVM) plays a crucial role in the modulation of pain. GABAergic neurons in the RVM (GABA neurons) facilitate nociceptive transmission by inhibiting off-cells activity.