A comparison of three rapid and accurate bioluminescent antibiotic susceptibility tests.

Introduction: Rapid determination of microorganisms' antibiotic susceptibility remains a great challenge for the clinical diagnosis of infectious diseases. In this study, we compare three rapid bioluminescent antibiotic susceptibility tests with the conventional normalised microdilution method.

Methods: The susceptibility of two bacterial and two fungal strains to a 6h incubation with one of eight standard antibiotics was investigated by the ATP, AK and QNO bioluminescent methods.

Antimicrobial activities of 3-amino- and polyaminosterol analogues of squalamine and trodusquemine.

A series of 3-amino- and polyaminosterol analogues of squalamine and trodusquemine were synthesized and evaluated for their in vitro antimicrobial properties against human pathogens. The activity was highly dependent on the structure of the different compounds involved and the best results were obtained with aminosterol derivatives 4b, 4e, 8b, 8e and 8n exhibiting minimum inhibitory concentrations (MICs) against yeasts, Gram positive and Gram negative bacteria at average concentrations of 3.12-12.

Squalamine: an appropriate strategy against the emergence of multidrug resistant gram-negative bacteria?

We reported that squalamine is a membrane-active molecule that targets the membrane integrity as demonstrated by the ATP release and dye entry. In this context, its activity may depend on the membrane lipid composition. This molecule shows a preserved activity against bacterial pathogens presenting a noticeable multi-resistance phenotype against antibiotics such as polymyxin B.

New stereoselective titanium reductive amination synthesis of 3-amino and polyaminosterol derivatives possessing antimicrobial activities.

A series of 3-amino and polyaminosterol analogues of squalamine and trodusquemine were synthesized involving a new stereoselective titanium reductive amination reaction in high chemical yields of up to 95% in numerous cases. These derivatives were evaluated for their in vitro antimicrobial properties against human pathogens. Activity was highly dependent on the different compounds' structures involved and best results have been obtained with aminosterol derivatives 4b, 4e and 6i exhibiting activities against yeasts, Gram positive and Gram negative bacteria at average concentrations of 6.

Synthesis of oxysterols and nitrogenous sterols with antileishmanial and trypanocidal activities.

Two sterol families have been synthesized: the first one is nitrogenous sterols containing amino, N-hydroxyimino or cyano group and the second one is oxysterols such as ketosterol and hydroxysterols. These compounds were then evaluated in vitro against Leishmania donovani promastigotes and Trypanosoma brucei brucei trypomastigotes. The most active compounds against L.