Serotyping and further characterization of group B streptococcal isolates from Indonesia.

Serotyping of 103 streptococci of serological group B isolated from human clinical specimens in Indonesia revealed mainly type pattern Ia/c and type patterns III and V, either alone or in combination with protein antigens c or R. Few cultures had serotype II and IV, none of the cultures had type antigen Ib or X. Extracts of one group B streptococcal culture reacted with antibodies specific to type antigens II, IV and R and one culture with antibodies specific to candidate type 7271.

Antibiotic resistance patterns and pigment production of streptococci of serological group B isolated from bovines and humans.

All 127 streptococci of serological group B isolated from bovines and humans were resistant to bacitracin (0.04 U) and gentamicin and susceptible to penicillin. Of the bovine isolates examined 10%, 8%, 22%, 2% and 30% were resistant to bacitracin (10 U), cefoxitin, clindamycin, erythromycin and tetracycline respectively.

Protein X of Streptococcus agalactiae induces opsonic antibodies in cows.

Protein X of Streptococcus agalactiae is a surface protein frequently associated with strains isolated from cases of mastitis of dairy cows. By immunizing cows with purified protein X, we obtained an antibody response which was restricted to X-bearing strains of S. agalactiae in a whole-cell enzyme-linked immunosorbent assay.

Purification of the protein X of Streptococcus agalactiae with a monoclonal antibody.

The protein X of Steptococcus agalactiae is a surface antigen included in the typing scheme of group B streptococci (GBS). We have developed a monoclonal antibody to the protein X and used it to purify this antigen by affinity chromatography. Electrophoresis in polyacrylamide, and immunoblotting using the monoclonal antibody or a rabbit antiserum raised with the affinity purified protein X, revealed a major band in the region of 200 kDa and a smaller one at 100 kDa.

The kinetics of inflammation and phagocytosis during bovine mastitis induced by Streptococcus agalactiae bearing the protein X.

The protein X of Streptococcus agalactiae is a surface antigen borne by a high proportion of strains isolated from bovine mastitis. We have tested the capacity of two strains of X-bearing Streptococcus agalactiae to induce mastitis in dairy cows. The reference X-strain (411.