Extracellular matrix re-normalization to improve cold tumor penetration by oncolytic viruses.

Immunologically inert or cold tumors pose a substantial challenge to the effectiveness of immunotherapy. The use of oncolytic viruses (OVs) to induce immunogenic cell death (ICD) in tumor cells is a well-established strategy for initiating the cancer immunity cycle (CIC). This process promotes the trafficking and infiltration of CD8+ T cells into tumors, thereby eliciting a tumor-specific immune response.

Non-viral, high throughput genetic engineering of primary immune cells using nanostraw-mediated transfection.

Transfection of proteins, mRNA, and chimeric antigen receptor (CAR) transgenes into immune cells remains a critical bottleneck in cell manufacturing. Current methods, such as viruses and bulk electroporation, are hampered by low transfection efficiency, unintended transgene integration, and significant cell perturbation. The Nanostraw Electro-actuated Transfection (NExT) technology offers a solution by using high aspect-ratio nanostraws and localized electric fields to precisely deliver biomolecules into cells with minimal disruption.

Hydrogen Evolution Reaction in Borophene: Puckering Mechanism and the Role of Coordination Number.

Using the free energy of hydrogen adsorption (Δ ) as the indicator, five borophene phases are previously shown to possess high catalytic activity for the hydrogen evolution reaction (HER). On these borophene phases, we investigate the role of the coordination number (CN = 4, 5, 6) of the adsorption sites and the puckering of the adsorption site. CN is discovered to have a profound effect on the Δ distribution, charge, and puckering height () of adsorption sites.

Gait speed assessment in confined spaces: Development of a novel automated 4-m static-start test to measure dynamic-start gait speed.

Objectives: To promote standardization and feasible measurements of gait speed across the field, we developed a device that used light detection and ranging (LiDAR) technology to measure gait speed from a standing-start testing procedure conducted on a 4-m total walkway. We compared this automated (LiDAR-based) standing-start 4-m gait speed test (AS-4MG) with automated and manual (stopwatch-based) dynamic-start 4-m gait speed tests (AD-4MG and MD-4MG, respectively) on between-method agreement, measurement repeatability, and predictive validity with functional outcomes.

Methods: A sample of 48 community-dwelling adults (mean [SD], 69 [9] years) participated in this method comparison study.