Publications by authors named "Y Kushida"
Article Synopsis
- Researchers identified Muse cells, a type of pluripotent stem cell, which play a role in tissue repair and are found in higher numbers in patients with acute myocarditis compared to controls.
- In biopsies from 17 patients with fulminant myocarditis, there were significantly more Muse cells present, indicating their potential involvement in the severity of myocardial injury.
- The study suggests that these Muse cells might help in healing damaged heart tissue and could correlate with clinical outcomes during both acute and recovery phases of myocarditis.
Background: Stanford type B-acute aortic dissection (type B-AAD) is often life-threatening without invasive surgery. Multilineage-differentiating stress enduring cell (Muse cells), which comprise several percent of mesenchymal stem cells (MSCs), are endogenous pluripotent-like stem cells that selectively home to damaged tissue and replace damaged/apoptotic cells by in-vivo differentiation.
Methods: Mortality, aortic diameter expansion, cell localization, cell differentiation, and inflammation of the dissected aorta were evaluated in type B-AAD model mice intravenously injected with human-Muse cells, -elastin-knockdown (KD)-Muse cells, -human leukocyte antigen-G (HLA-G)-KD-Muse cells, or MSCs, all without immunosuppressant.
Article Synopsis
- Muse cells are pluripotent-like stem cells found in bone marrow, peripheral blood, and organ connective tissues, identified by the SSEA-3 marker, though their specifics in extraembryonic tissue are less understood.
- Human umbilical cord SSEA-3(+) cells show characteristics that resemble early-stage development, expressing pluripotency markers and differentiating efficiently at the single-cell level, unlike adult tissue Muse cells.
- The discovery that human-UC-SSEA-3(+) cells bear a gene expression profile similar to post-implantation blastocysts, along with their potential for lower differentiation-related DNA methylation, suggests they could serve as important resources for research in human development and reproductive medicine.
Background: The current method for generating an animal model of spinal cord (SC) infarction is highly invasive and permits only short-term observation, typically limited to 28 days.
Objective: We aimed to establish a rat model characterised by long-term survival and enduring SC dysfunction by inducing selective ischaemic SC damage.
Methods: In 8-week-old male Wistar rats, a convection-enhanced delivery technique was applied to selectively deliver endothelin-1 (ET-1) to the anterior horn of the SC at the Th13 level, leading to SC infarction.
Article Synopsis
- Bleomycin-induced lung injury leads to inflammation and fibrosis, similar to conditions like idiopathic pulmonary fibrosis, and stem cell therapy is being explored as a treatment option.
- Multilineage-differentiating stress-enduring (Muse) cells, which are stem cells found in various tissues, can target and repair damaged lung tissue without the need for immunosuppressants.
- In a rat study, those treated with Muse cells from preterm and term umbilical cords showed significant improvements in recovery metrics such as weight, serum levels, and lung injury scores compared to controls.