Publications by authors named "Y Kuriki"
Carboxypeptidases (CPs) are a family of hydrolases that cleave one or more amino acids from the C-terminal of peptides or proteins and play indispensable roles in various physiological and pathological processes. However, only a few highly activatable fluorescence probes for CPs have been reported, and there is a need for a flexibly tunable molecular design platform to afford a range of fluorescence probes for CPs for biological and medical research. Here, we focused on the unique activation mechanism of ProTide-based prodrugs and established a modular design platform for CP-targeting florescence probes based on ProTide chemistry.
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- The study investigates the effectiveness of measuring pancreatic chymotrypsin activity as a method to predict postoperative pancreatic fistula (POPF) more accurately than the traditional amylase level assessment.
- In 52 patients undergoing pancreaticoduodenectomy, chymotrypsin activity was analyzed and found to have higher predictive value for clinically relevant POPF (CR-POPF) compared to amylase levels.
- The findings suggest that using pancreatic chymotrypsin activity leads to timely and accurate identification of CR-POPF, allowing for better drain management by surgeons right at the patient's bedside.
Article Synopsis
- Complete resection is the only effective treatment for extrahepatic cholangiocarcinoma (eCCA), but existing imaging methods struggle to accurately diagnose its spread.
- Researchers developed a fluorescence imaging technique using an enzyme-activatable probe that helps identify eCCA in real-time during clinical procedures.
- The most effective probe discovered, PM-2MeSiR, showed high diagnostic accuracy by matching cancerous regions found in tissue samples, with potential activation by the enzyme puromycin-sensitive aminopeptidase.
Irritable bowel syndrome (IBS) has no clinically accepted biomarkers even though it affects a large number of individuals worldwide. To address this lack of understanding, we evaluated peptidase activity in fecal samples from 35 patients with diarrheal IBS without symptom exacerbation (IBS-n) and 35 healthy subjects using a library of 384 fluorescent enzymatic substrate probes. IBS-n patients had high trypsin-like peptidase activity for cleavage of C-terminal lysine and arginine residues and low elastase-like activity for cleavage of C-terminal serine and glycine residues.
View Article and Find Full Text PDFFluorescent probes that can selectively detect tumour lesions have great potential for fluorescence imaging-guided surgery. Here, we established a library-based approach for efficient screening of probes for tumour-selective imaging based on discovery of biomarker enzymes. We constructed a combinatorial fluorescent probe library for aminopeptidases and proteases, which is composed of 380 probes with various substrate moieties.
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