Publications by authors named "Y Krishna Jyothi"
Background And Aim: Bluetongue (BT) is a major disease of sheep and goats and is endemic to India. It is known to cause significant economic losses to the sheep industry. The current study aimed to determine the type-specific seroprevalence of BT in sheep population of India during 2018-2019.
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- Reassortment in segmented viruses allows co-infecting strains to exchange genetic segments, leading to new virus genotypes, but its effects on transmission and diversity are not well understood.
- The study focused on the bluetongue virus (BTV), analyzing 92 genomes from India over four decades, revealing frequent reassortment and selective pressures acting on the virus.
- Findings showed a recent selective sweep on segment 5's NS1 protein driving a single variant's dominance, while diversifying selection maintained genetic diversity in other essential surface protein genes, supporting the idea that reassortment contributes to rapid changes in virus traits.
Aim: The present study was designed to standardize real-time polymerase chain reaction (PCR) for detecting the bluetongue virus from blood samples of sheep collected during outbreaks of bluetongue disease in the year 2014 in Andhra Pradesh and Telangana states of India.
Materials And Methods: A 10-fold serial dilution of Plasmid PUC59 with bluetongue virus (BTV) NS3 insert was used to plot the standard curve. BHK-21 and KC cells were used for propagation of virus BTV-9 at a TCID50/ml of 10 ml and RNA was isolated by the Trizol method.
The hexane extract of oleo-gum-resin of Boswellia serrata (BSHE) was evaluated for its effect on liver injury induced by carbon tetrachloride, paracetamol or thioacetamide. The BSHE was given in two different doses (87.5 mg/kg p.
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