Publications by authors named "Y Kozawa"

Single-shot laser ablation is performed on the surface of a transparent glass material using a radially polarized femtosecond beam. Theoretical and experimental investigation revealed the significant role of the material interface under high-numerical-aperture conditions. The longitudinal electric field at the focus was remarkably enhanced due to the total reflection on the interface when a radially polarized beam was focused on the back surface of the glass from the inside using an immersion lens.

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High-speed three-dimensional (3D) imaging is essential for revealing the structure and functions of biological specimens. Confocal laser scanning microscopy has been widely employed for this purpose. However, it requires a time-consuming image-stacking procedure.

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This study presents an alternative approach for two-photon volumetric imaging that combines multibeam lateral scanning with continuous axial scanning using a confocal spinning-disk scanner and an electrically focus tunable lens. Using this proposed system, the brain of a living mouse could be imaged at a penetration depth of over 450 μm from the surface. volumetric Ca imaging at a volume rate of 1.

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Article Synopsis
  • Cystic fibrosis (CF) is caused by mutations in the CFTR gene and is more common in Caucasians than in East Asians; this study investigated CF characteristics in Japanese patients.
  • The analysis of 132 CF patients revealed prevalent symptoms such as chronic lung disease (85.6%) and pancreatic insufficiency (66.7%), with a median survival age of just 25 years.
  • The study found distinct CFTR variant patterns in Japanese patients compared to European ones, with specific variants like CFTR-dele16-17a-17b being more common in Japan and the F508del variant appearing in European alleles.
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We demonstrate the femtosecond-laser processing of self-suspended monolayer graphene grown by chemical vapor deposition, resulting in multipoint drilling with holes having a diameter of <100 nm. Scanning transmission electron microscopy revealed the formation of many nanopores on the laser-irradiated graphene. Furthermore, atomic-level defects as well as nanopores were found in the graphene membrane by high-resolution transmission electron microscopy, while the overall crystal structure remained intact.

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