Single-shot laser ablation is performed on the surface of a transparent glass material using a radially polarized femtosecond beam. Theoretical and experimental investigation revealed the significant role of the material interface under high-numerical-aperture conditions. The longitudinal electric field at the focus was remarkably enhanced due to the total reflection on the interface when a radially polarized beam was focused on the back surface of the glass from the inside using an immersion lens.
View Article and Find Full Text PDFHigh-speed three-dimensional (3D) imaging is essential for revealing the structure and functions of biological specimens. Confocal laser scanning microscopy has been widely employed for this purpose. However, it requires a time-consuming image-stacking procedure.
View Article and Find Full Text PDFThis study presents an alternative approach for two-photon volumetric imaging that combines multibeam lateral scanning with continuous axial scanning using a confocal spinning-disk scanner and an electrically focus tunable lens. Using this proposed system, the brain of a living mouse could be imaged at a penetration depth of over 450 μm from the surface. volumetric Ca imaging at a volume rate of 1.
View Article and Find Full Text PDFWe demonstrate the femtosecond-laser processing of self-suspended monolayer graphene grown by chemical vapor deposition, resulting in multipoint drilling with holes having a diameter of <100 nm. Scanning transmission electron microscopy revealed the formation of many nanopores on the laser-irradiated graphene. Furthermore, atomic-level defects as well as nanopores were found in the graphene membrane by high-resolution transmission electron microscopy, while the overall crystal structure remained intact.
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