Morphotype-specific calcium signaling in human microglia.

Background: Key functions of Ca signaling in rodent microglia include monitoring the brain state as well as the surrounding neuronal activity and sensing the danger or damage in their vicinity. Microglial Ca dyshomeostasis is a disease hallmark in many mouse models of neurological disorders but the Ca signal properties of human microglia remain unknown.

Methods: We developed a novel genetically-encoded ratiometric Ca indicator, targeting microglial cells in the freshly resected human tissue, organotypically cultured tissue slices and analyzed in situ ongoing Ca signaling of decades-old microglia dwelling in their native microenvironment.

Layered double hydroxide nanoparticles for a smartphone digital image colorimetry-based determination of fluoride ions in water, milk and dental products.

A novel smartphone digital image colorimetry-based approach was developed for fluoride ion determination, using layered double hydroxide (LDH) nanoparticles (MgAl(OH)) as sorbents for analyte preconcentration and as a source of a central metal ion for a colored complex formation. A cotton rotating disk provided effective retention of the LDH nanoparticles during solid-phase microextraction and elution, excluding time-consuming centrifugation process. In the proposed procedure, the nanoparticles acted as aluminum ion source for alizarin complexone-Al-F complex formation.

CLINICAL, INSTRUMENTAL AND BIOCHEMICAL ASSOCIATIONS OF THE DEGREE OF PHYSICAL RECOVERY IN PATIENTS SUFFERED FROM ACUTE CORONARY SYNDROME AT THE COMPLETION OF IN-HOSPITAL REHABILITATION PERIOD.

Objective: The aim: To determine the clinical, instrumental and biochemical factors associated with the degree of physical recovery in patients suffered from acute coronary syndrome (ACS) at the completion of in-hospital rehabilitation period.

Patients And Methods: Materials and methods: We enrolled 88 patients (all were males); 77 patients had ACS/STEMI and 11 with ACS/unstable angina. The mean age was (median, interquartile range) was 58 (49-64) years.

Neuronal silence as a prosurvival factor for adult-born olfactory bulb interneurons.

Adult-born cells, arriving daily into the rodent olfactory bulb, either integrate into the neural circuitry or get eliminated. However, whether these two populations differ in their morphological or functional properties remains unclear. Using longitudinal in vivo two-photon imaging, we monitored dendritic morphogenesis, odor-evoked responsiveness, ongoing Ca signaling, and survival/death of adult-born juxtaglomerular neurons (abJGNs).

Impact of Vitamin D3 on Carbonyl-Oxidative Stress and Matrix Metalloproteinases after Acute Intracerebral Hemorrhage in Rats with Type 2 Diabetes Mellitus.

Introduction: Diabetes mellitus is associated with the development of carbonyl-oxidative stress (COS) and an increased risk of a cerebral hemorrhage. Vitamin D3 is considered an additional drug to have an impact on COS and proteolysis in the extracellular matrix.

Objective: The study aimed to evaluate the impact of D3 on the COS-markers and matrix metalloproteinases MMP2/MMP9 activity after acute intracerebral hemorrhage (ICH) in rats with experimental type 2 diabetes mellitus (Т2DM) compared to metformin (Met).