Salt-inducible kinase 2 confers radioresistance in colorectal cancer by facilitating homologous recombination repair.

Resistance to radiotherapy remains a critical barrier in treating colorectal cancer (CRC), particularly in cases of locally advanced rectal cancer (LARC). To identify key kinases involved in CRC radioresistance, we employed a kinase-targeted CRISPR-Cas9 library screen. This approach aimed to identify potential kinase inhibitors as radiosensitizers.

Three-dimensional single-pixel imaging using a fractal superconducting nanowire single-photon detector.

Faint-light imaging plays an important role in applications including fluorescence-lifetime microscopy and remote sensing. Superconducting nanowire single-photon detectors (SNSPDs) outperform other single-photon detectors in terms of comprehensive performance, however, large-format SNSPD imagers with many pixels remain an outstanding technological challenge. Here, as an alternative route, we use a multimode-fiber-coupled fractal SNSPD as the light-sensing element to perform three-dimensional single-pixel imaging at the wavelength of 1560 nm.

Light-up lipid droplets dynamic behaviors using rationally designed carbon dots.

Lipid droplets (LDs) are essential organelles used to store lipids and participate in cellular lipid metabolism. Imaging LDs is an intuitive approach to comprehend their biological functions. Herein, the LDs-targeted CDs (LD-CDs) featuring robust solvatochromic emission were elaborately designed by a Schiff base reaction using 1, 2-diamino-4-fluorobenzene, 3-dimethylaminophenol, and thiourea as precursors.

Efficacy and Safety of Proactive Therapy with 2% Crisaborole Ointment in Children with Mild-to-Moderate Atopic Dermatitis: A Randomized Controlled Study.

Background: The effectiveness of crisaborole for proactive treatment of atopic dermatitis (AD) is not well established.

Objectives: This study aims to investigate the efficacy and safety of a proactive treatment strategy with 2% crisaborole ointment for managing mild-to-moderate AD in children.

Patients And Methods: In this 16-week randomized-controlled trial, children aged 2-17 years with mild-to-moderate AD were enrolled.

Effect of transgene on salt tolerance of tobacco.

To explore the effects of salt-tolerance gene accumulation on salt tolerance in transgenic plant, we used four types of plant expression vector (N27, N28, N29, and N30) carrying mtlD, mtlD + gutD, mtlD + gutD + BADH, mtlD + gutD + BADH + sacB genes respectively, to transform tobacco through Agrobacterium-mediated method. Transgenic lines were identified through polymerase chain reaction (PCR) detection. Transgenic lines and non-transgenic plant (CK) were subjected to 6‰ sodium chloride solution stress; then, fluorescence quantitative PCR (FQ-PCR) and salt tolerance indexes were used to assess characteristics.