Publications by authors named "Y Inage"
Article Synopsis
- * This study developed methods for obtaining pig fetal kidneys through cesarean sections and long-term cryopreservation, ensuring the health of the mother pig.
- * Results showed that cryopreserved pig fetal kidneys could grow and form organoids after being grafted into mice, indicating a promising approach for advancing xenotransplantation research.
Purpose: This study aimed to compare the degree of maturation and development of fetal pig segmental intestinal tissue with that of spheroids created by in-vitro reaggregation of dissociated fetal intestinal cells after transplantation into immunodeficient mice.
Methods: Fetal pig small intestines were transplanted as segmental grafts into the omentum and subrenal capsules of immunodeficient mice or enzymatically treated to generate single cells. Spheroids made by in-vitro reaggregation of these cells were transplanted into the subrenal capsules of immunodeficient mice.
Article Synopsis
- Human pluripotent stem cells are used to create organoids, but they pose challenges due to their immaturity and limited cell types compared to fetal organs.
- The study investigates preservation methods for porcine fetal kidneys, hearts, and livers, focusing on their potential use in regeneration and xenotransplantation.
- Results show that while kidney cells can differentiate effectively after freezing, heart cells perform better when frozen post-dissociation, and liver cells can be preserved using a new perfusion technique, enhancing their viability for research.
Kidney organoids have shown promise as evaluation tools, but their in vitro maturity remains limited. Transplantation into adult mice has aided in maturation; however, their lack of urinary tract connection limits long-term viability. Thus, long-term viable generated nephrons have not been demonstrated.
View Article and Find Full Text PDFThe number of patients with end-stage renal failure is increasing annually worldwide and the problem is compounded by a shortage of renal transplantation donors. In our previous research, we have shown that transplantation of renal progenitor cells into the nephrogenic region of heterologous fetuses can induce the development of nephrons. We have also developed transgenic mice in which specific renal progenitor cells can be removed by drugs.
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