Identifying the best reference gene for RT-qPCR analyses of the three-dimensional osteogenic differentiation of human induced pluripotent stem cells.

Reverse transcription quantitative real-time polymerase chain reaction (RT-qPCR) is an essential tool for gene expression analysis; choosing appropriate reference genes for normalization is crucial to ensure data reliability. However, most studies on osteogenic differentiation have had limited success in identifying optimal reference genes. To the best of our knowledge, no optimal reference genes in three-dimensional (3D) osteogenic differentiation culture experiments using human induced pluripotent stem cells (hiPSCs) have been identified.

Efficacy of the Bridge Dry Swallowing Exercise for Refractory Gastroesophageal Reflux Disease.

Background Gastroesophageal reflux disease (GERD) is a condition characterized by reflux of gastric contents, leading to damage to the esophageal mucosa and/or unpleasant symptoms, with a global prevalence of 13%. Although acid-suppressive medications are currently the most effective treatments for GERD, they may be ineffective against its symptoms. We therefore investigated the efficacy of a physical exercise program for the treatment of intractable GERD symptoms.

The Influence of Total Fat Mass and Skeletal Muscle Mass Index on the Occurrence of Perioperative Hypothermia in Patients Undergoing Open Gastrectomy.

Background: Perioperative hypothermia, a common occurrence in patients undergoing general anesthesia, is defined as a core body temperature below 36°C. The relationship between patient body composition and the incidence of hypothermia remains underreported. This study aims to elucidate the association between body composition and perioperative hypothermia in patients undergoing open gastrectomy.