Simultaneous Detection of Multiple Analytes at Ambient Temperature Using Eukaryotic Artificial Cells with Modular and Robust Synthetic Riboswitches.

Cell-free systems, which can express an easily detectable output (protein) with a DNA or mRNA template, are promising as foundations of biosensors devoid of cellular constraints. Moreover, by encasing them in membranes such as natural cells to create artificial cells, these systems can avoid the adverse effects of environmental inhibitory molecules. However, the bacterial systems generally used for this purpose do not function well at ambient temperatures.

Single-Sided Delocalized Polarization of the C Cage and Reduced Infrared Intensities and Dipole Moment of HO@C.

The C fullerene cage can encapsulate a small molecule like water and provides room to leave the encapsulated component rather isolated, but the true nature of the intracomplex interactions should be further elucidated for better understanding and utility of this series of complexes. Here, an analysis toward this goal is conducted for HO@C by infrared spectral measurements and theoretical calculations. It is shown that the response of the π electrons of the C cage upon encapsulating a water molecule is single-sided and delocalized in that the electron density is partially transferred from the - side to the + side of the cage (when the axis is taken along the water dipole) but almost only inside the cage, explaining the significant reduction of the dipole moment and the infrared intensities.

A van der Waals porous crystal featuring conformational flexibility and permanent porosity for ultrafast water release.

Flexibility has been pursued enthusiastically in the field of porous crystals for enhancing their adsorption and separation performances. However, flexibility has never been observed among porous crystals sustained thoroughly by van der Waals interactions since flexible motions readily lead to the collapse of the porous architecture. Here we report a van der Waals crystal featuring conformational flexibility as well as permanent microporosity.

Proton transfer driven by the fluctuation of water molecules in chitin film.

Proton-transfer mechanisms and hydration states were investigated in chitin films possessing the functionality of fuel-cell electrolytes. The absolute hydration number per chitin molecule (N) as a function of relative humidity (RH) was determined from the OH stretching bands of H2O molecules, and the proton conductivity was found to enhance above N = 2 (80%RH). The FIR spectrum at 500-900 cm-1 for 20%RH (N < 1) together with first-principles calculations clearly shows that the w1 site has the same hydration strength as the w2 site.

Hyperconfined bio-inspired Polymers in Integrative Flow-Through Systems for Highly Selective Removal of Heavy Metal Ions.

Access to clean water, hygiene, and sanitation is becoming an increasingly pressing global demand, particularly owing to rapid population growth and urbanization. Phytoremediation utilizes a highly conserved phytochelatin in plants, which captures hazardous heavy metal ions from aquatic environments and sequesters them in vacuoles. Herein, we report the design of phytochelatin-inspired copolymers containing carboxylate and thiolate moieties.