Superficial wounding model for epidermal barrier repair studies: comparison of Erbium:YAG laser and the suction blister method.

Background And Objectives: Wound-healing studies use mainly mechanical methods for wound induction, which are laborious and difficult to standardize. Objective of this study was to evaluate the Erbium:Yttrium-Aluminium-Garnet (Er:YAG) laser method as a model of epidermis ablation on human skin in vivo and to compare the quality and healing rates of Er:YAG laser and suction blister (SB) wounds.

Materials And Methods: Er:YAG laser and SB wounds were made on the forearms of 10 healthy volunteers.

A multitechnique evaluation of topical corticosteroid treatment.

Background/purpose: Corticosteroids are widely prescribed for systemic or local treatment of inflammatory autoimmune disorders. Long-term therapy is associated with side effects and causes cutaneous atrophy of the epidermis and the dermis. The present study aims to evaluate with several noninvasive techniques, the skin modifications observed during corticosteroids treatment.

Use of a 3-D imaging technique for non-invasive monitoring of the depth of experimentally induced wounds.

Background/purpose: Experimental studies of wound healing lack methods for standardized wounding and in situ depth assessment. Consequently, in this pilot study, an Erbium (Er):YAG laser has been used for wound induction with a non-invasive 3-D imaging technique as an alternative to histology.

Methods: Erbium:YAG ablation of human skin ex vivo was performed with total fluences of 10, 50 and 200 J/cm(2), removing the stratum corneum, epidermis/papillary dermis and deeper dermis, respectively.