Development of a triplex qPCR system for the detection of and from wheat.

A triplex qPCR system was developed for the simultaneous detection of the three most prevalent wheat leaf spot diseases: septoria nodorum blotch caused by Parastagonospora nodorum, septoria tritici blotch caused by Zymoseptoria tritici, and tan spot caused by Pyrenophora tritici-repentis. In this system, the primer set for P. tritici-repentis targets a species-specific multicopy genomic region, while the primer sets for the other two pathogens target the ribosomal DNA (rDNA) region.

A practical synthesis of YZD-7082B, a novel orally bioavailable selective estrogen receptor degrader (SERD) for the treatment of ER+ breast cancer.

In previous studies, we discovered YZD-7082B, a selective estrogen receptor degrader (SERD) with excellent comprehensive properties. Here, we reported the development of an efficient multigram-scale synthetic process for YZD-7082 in 13 steps. The route featured a chiral resolution of a thiochroman intermediate with a unique cis-1,2-diaryl motif using a chiral amine and a mild reduction of amide using ZnEt/LiCl/(EtO)SiH system.

Single-center retrospective study on the clinical application of multi-arm robotic-assisted uniportal video-assisted thoracic surgery.

This research aims to consolidate clinical experiences with hybrid multi-arm robotic-assisted uniportal thoracoscopic surgery (H-URATS) employing endoscopic staplers and assess its safety and feasibility. A retrospective review was conducted on the clinical records of 128 patients treated with H-URATS at the Department of Thoracic Surgery II, Xinjiang Medical University Affiliated Tumor Hospital, between January and December 2024. The cohort included 52 men and 76 women, with an average age of 57.

Preparation of Hybrid Molecularly Imprinted Polymers Based on 3-Triethoxysilylpropyl Methacrylic Amide for Solid-Phase Extraction of Gatifloxacin From Lake Water.

A novel molecularly imprinted polymer (MIP) against gatifloxacin based on a hybrid monomer (3-triethoxysilylpropyl methacrylic amide, APTES-MAA) was reported. In this study, the imprinted monolith was synthesized with gatifloxacin as template, ethylene glycol dimethacrylate (EGDMA) as cross-linker, and APTES-MAA as functional monomer in a mixture of acetonitrile and isooctane as porogen via an optimization based on density functional theory (DFT). The polymers were characterized by scanning electron microscopy, Fourier transform infrared spectroscopy, and mercury porosimetry.

Integration of Circulating Tumor DNA and Metabolic Parameters on F-Fludeoxyglucose Positron Emission Tomography for Outcome Prediction in Unresectable Locally Advanced Non-Small Cell Lung Cancer.

This prospective study explores the prognostic value of circulating tumor DNA (ctDNA) and positron emission tomography/computed tomograpy (PET/CT) in unresectable locally advanced non-small cell lung cancer (LA-NSCLC) treated with definitive chemoradiotherapy (CRT). The discovery set includes 62 patients, with 62 baseline and 53 post-CRT plasma samples. PET/CT is performed at baseline, and 33 patients undergo mid-treatment scans after 40 Gy.