Transport stress increases somatic cell counts in milk, and enhances the migration capacity of peripheral blood neutrophils of dairy cows.

The present study was designed to determine the effects of physiological stress on milk-somatic cell counts (SCC) and function of bovine peripheral blood leukocytes (PBL). Nine healthy lactating cows were used in the examination. Five cows were transported 100 km for 4 hr (transported group; TG), and 4 cows were penned (non-transported group; NTG).

Accelerated binding of autoantibody to red blood cells with increasing anaemia in cattle experimentally infected with Theileria sergenti.

Anaemia is the most important clinical manifestation in cattle infected with Theileria sergenti. In order to determine the mechanism of red blood cells (RBC) destruction in anaemic cattle, we studied the binding of autoantibody (IgG) to RBC during the development of anaemia in T. sergenti infection.

Cytokine and inducible nitric oxide synthase gene expressions in peripheral blood mononuclear cells and related clinical characteristics in Theileria orientalis sergenti-infected calves.

Eight splenectomized calves were inoculated with Theileria orientalis sergenti (Tos)-infected tick gland homogenate (5 calves) or infected erythrocyte suspension (3 calves). Clinical characteristics were different in calves post-infection. Animals were divided into 3 groups on the basis of susceptibility as high, middle, and low.

The influence of oxidative bursts of phagocytes on red blood cell oxidation in anemic cattle infected with Theileria sergenti.

The primary clinical symptom of Japanese bovine theileriosis, caused by the intraerythrocytic protozoan Theileria sergenti, is anemia, but the underlying mechanism of this anemia remains unknown. To elucidate the pathogenesis of anemia developing in bovine theileriosis, we investigated the relationship between oxidative bursts of peripheral blood phagocytes (neutrophils and monocytes) and the oxidation of red blood cells (RBC) to the development of anemia in cattle experimentally infected with T. sergenti.

Oxidative damage and phosphatidylserine expression of red blood cells in cattle experimentally infected with Theileria sergenti.

In order to elucidate the mechanism of anemia in Japanese bovine theileriosis, we investigated the oxidative alteration of red blood cells (RBCs) in cattle infected with Theileria sergenti. As an index of RBC oxidation, the levels of 2',7'-dichlorofluorescin-diacetate (DCFH) oxidation and malondialdehyde-thiobarbituric acid reactive substances (MDA-TBARS), and phosphatidylserine (PS) expression accompanying anemia were examined in experimentally infected cattle. Before the development of anemia, the concentrations of DCFH oxidation and MDA-TBARS were low, and PS expression on the surfaces of RBCs was hardly seen.