Publications by authors named "Y Chau"
Inefficient endosomal escape has been regarded as the main bottleneck for intracellular nucleic acid delivery. While most research efforts have been spent on designing various nano-sized particles, we took a different path here, investigating micron-sized carriers for direct cytosol entry. Using the spontaneous co-assembly of mRNA and the designer 27 amino acid oligopeptide named pepMAX2, micron-sized co-assemblies were obtained with various sizes by altering the concentration of NaCl salt and time for pre-incubation.
View Article and Find Full Text PDFNiobium carbide (NbCTx), a key component of the MXene family renowned for its utilization in lithium-ion batteries and supercapacitors, remains largely underutilized in biosensing applications. This study introduces a notably sensitive and label-free dual-mode electrochemiluminescence (ECL) and colorimetric immunosensor to specifically detect chromogranin A (CgA) in biological fluids. Initially, AuAg bimetallic nanoparticles (BiMNPs) were synthesized using NbCTx as a reducing and supporting material.
View Article and Find Full Text PDFArticle Synopsis
- * Mutations in Rab23, such as M12K, C85R, and Y79del, occur within its GTPase domain and affect its function, but the exact activation mechanisms and how these mutations lead to disease are not fully understood.
- * High-resolution crystal structures of human Rab23 and the Y79del mutant have been determined, showing that the deletion leads to structural changes that disrupt its interactions with partners, contributing to its loss of function in Carpenter Syndrome.
Fused in sarcoma (FUS) is an intrinsically disordered RNA-binding protein that helps to regulate transcription and RNA transport while reversibly assembling into membraneless organelles (MLOs). Some mutations of FUS can promote irreversible aggregation, contributing to neurodegenerative diseases. We previously reported a multi-scale computational framework combining a series of molecular dynamics simulations (MD) followed by lattice Monte Carlo (MC) simulations to describe the tendency and dynamics of the assembly and disassembly of intrinsically disordered proteins (IDPs) using wild-type (WT)-FUS as an illustrative example.
View Article and Find Full Text PDFBackground/objectives: There has been limited evidence on the long-term impacts of coffee intake on health. We aimed to investigate the association between coffee intake and the incidence of diseases and mortality risk over 20 years among community-dwelling Chinese adults.
Methods: Participants were from the Hong Kong Osteoporosis Study who attended baseline assessments during 1995-2010.