Publications by authors named "Y Ann Chen"
Here, we present a protocol for conditional mutagenesis in zebrafish germ cells using Tol2 transposon and a CRISPR-Cas9-based plasmid system. We describe steps for conditional mutagenesis plasmid construction, zebrafish embryo microinjection, and screening for green fluorescence in the heart. This protocol is simple to execute, time efficient, and multifunctional, enabling the disruption of genes in zebrafish germ cells to be conducted with ease.
View Article and Find Full Text PDFNovel multiple Z-score models for detection of coronary artery dilation: application in Kawasaki disease.
Pediatr Rheumatol Online J
December 2024
Background: This study aims to develop Z-Score models to normalize measurements of three coronary arteries and enhance the diagnosis of Kawasaki disease (KD) in children from newborns to 10 years old. Developing a reliable Z-Score model is challenging, as some existing models fail the normality test. Overcoming these challenges is crucial for improving KD diagnosis.
View Article and Find Full Text PDFPeriodontal ligament fibroblasts (PDLFs) play a crucial role in the etiology of periodontitis and periodontal tissue regeneration. In healthy periodontal tissues, PDLFs maintain the homeostasis of periodontal soft and hard tissues as well as the local immune microenvironment. PDLFs also have the potential for multidirectional transdifferentiation and are involved in periodontal tissue regeneration.
View Article and Find Full Text PDFArticle Synopsis
- Incessant ovulation contributes to ovarian high-grade serous carcinomas (HGSC), which primarily arise from the fallopian tube epithelium (FTE), and receptor tyrosine kinase (RTK) ligands play a key role in this process.
- A study investigated follicular fluid exosomes from women undergoing in vitro fertilization to identify RTK ligands and their impact on FTE cells, using various RTK inhibitors.
- The findings revealed that FF exosomes were rich in transformative abilities and essential EGFR ligands, promoting cell growth and migration, indicating their significant contribution to HGSC development.
Article Synopsis
- - Lenvatinib is a key treatment for hepatocellular carcinoma (HCC) that works by blocking critical growth factor receptors, which helps reduce tumor growth and improves immune response.
- - Although lenvatinib is effective on its own, drug resistance is a growing issue due to factors like genetic mutations and changes in the tumor environment.
- - Combining lenvatinib with immune checkpoint inhibitors could enhance treatment effectiveness, but challenges related to safety remain, highlighting the need for research on resistance mechanisms and biomarkers for better personalized therapies.