Philos Trans A Math Phys Eng Sci
July 2024
Optical projection tomography (OPT) is a three-dimensional mesoscopic imaging modality that can use absorption or fluorescence contrast, and is widely applied to fixed and live samples in the mm-cm scale. For fluorescence OPT, we present OPT implemented for accessibility and low cost, an open-source research-grade implementation of modular OPT hardware and software that has been designed to be widely accessible by using low-cost components, including light-emitting diode (LED) excitation and cooled complementary metal-oxide-semiconductor (CMOS) cameras. Both the hardware and software are modular and flexible in their implementation, enabling rapid switching between sample size scales and supporting compressive sensing to reconstruct images from undersampled sparse OPT data, e.
View Article and Find Full Text PDF'openFrame' is a modular, low-cost, open-hardware microscopy platform that can be configured or adapted to most light microscopy techniques and is easily upgradeable or expandable to multiple modalities. The ability to freely mix and interchange both open-source and proprietary hardware components or software enables low-cost, yet research-grade instruments to be assembled and maintained. It also enables rapid prototyping of advanced or novel microscope systems.
View Article and Find Full Text PDFNeurosci Biobehav Rev
June 2022
Current research on the molecular mechanisms of learning and memory is based on the "stimulus-response" paradigm, in which the neural circuits connecting environmental events with behavioral responses are strengthened. By contrast, cognitive and systems neuroscience emphasize the intrinsic activity of the brain that integrates information, establishes anticipatory actions, executes adaptive actions, and assesses the outcome via regulatory feedback mechanisms. We believe that the difference in the perspectives of systems and molecular studies is a major roadblock to further progress toward understanding the mechanisms of learning and memory.
View Article and Find Full Text PDFAnalytic and holistic thinking styles are known to be associated with individual differences in various aspects of behavior and brain activity. In this study, we tested a hypothesis that differences in thinking styles may also be manifested at the level of neuro-visceral coordination. Heart rate variability (HRV) was compared between analytic and holistic thinkers at rest, during a simple motor choice reaction time task and when solving cognitive choice reaction time tasks in conditions with varying instructions contrasting the role of the field when evaluating objects.
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