Regulatory role of nitric oxide in neutrophil apoptosis.

Apoptosis of blood neutrophils from healthy donors was studied under conditions of cell culturing with different concentrations of H(2)O(2), selective NO synthase inhibitor, and inductor of NO synthesis (L-arginine). In vitro incubation of neutrophilic leukocytes with 5 mM H(2)O(2) led to activation of the apoptotic program in neutrophils, which was seen from increased content of Bax protein in the cells and increased number of apoptotic cells in the culture. Increased content of annexin-positive cells after incubation of neutrophil culture with NO synthase inhibitor suggests involvement of NO in the regulation of neutrophil apoptosis under conditions of oxidative stress, while L-arginine prevented H(2)O(2)-induced programmed cell death.

[The lipid spectrum of erythrocyte membranes in children with diabetes mellitus].

The present study was undertaken to examine the lipid spectrum of erythrocytic membranes and serum in children and adolescents with type 1 diabetes mellitus (DM-1) during pathogenetic therapy in relation to the duration of the disease, its severity, and the presence of vascular events. Ninety-nine patients with DM-1 and 40 healthy children of the same age were examined. The patients were on basic bolus insulin therapy.