Publications by authors named "Xuyuan Gao"

Article Synopsis
  • * Using advanced sequencing techniques, researchers found that the major gut bacterial communities were from the phyla Proteobacteria and Bacteroidetes, with notable genera such as Providencia and Klebsiella.
  • * The presence of stress from the insecticide led to a decrease in certain bacteria (Citrobacter, Enterobacter, Klebsiella) and an increase in others (Providencia, Pectobacterium), indicating that insecticides significantly disrupt the gut microbiota of Z. cucurbitae.
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Objective: This systematic review and meta-analysis aims to investigate the effectiveness and safety of acupuncture and moxibustion in managing perioperative anxiety during gynecological surgery.

Methods: Relevant studies published from the establishment of the databases to March 20, 2023, were searched in PubMed, Embase, Cochrane Library, Web of Science, CNKI, Wanfang, VIP, and CBM. Literature screening and data extraction were independently conducted by two investigators.

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Extrachromosomal circular DNA (eccDNA) is crucial in oncogene amplification, gene transcription regulation, and intratumor heterogeneity. While various analysis pipelines and experimental methods have been developed for eccDNA identification, their detection efficiencies have not been systematically assessed. To address this, we evaluate the performance of 7 analysis pipelines using seven simulated datasets, in terms of accuracy, identity, duplication rate, and computational resource consumption.

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Successful bisexual reproduction requires interactions between males and females. Male-derived seminal fluid proteins (SFPs) transferred to females during mating profoundly affect females from pre- to post-mating, and the subsequent shift in female physiology enhances their fertility. SFPs have important evolutionary implications for the fitness of many insects.

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  • The leaf beetle Ophraella communa is a biological control agent for common ragweed, and researchers sequenced its genome using advanced technologies to enhance their study.
  • The complete genome assembly is 733.1 Mb in size with 17 chromosomes and contains 25,873 protein-coding genes, of which most have functional annotations.
  • Over half of the genome (57.76%) consists of repetitive elements, highlighting its complexity and providing crucial information for improving biological control methods that utilize this beetle.
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  • - The melon fly is an invasive pest that threatens agriculture in Africa and beyond, and its chemosensory system's molecular structure is not well understood.
  • - Researchers used next-generation sequencing to analyze the antennae of immature adult melon flies, identifying 160 potential chemosensory genes, including multiple types of receptors and proteins.
  • - Validation of the findings showed distinct expression patterns of certain genes in male and female flies, providing insights into insect olfaction that could inform better pest management strategies.
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The melon fly, Zeugodacus cucurbitae (Coquillett), is a major invasive pest, widely distributed in the Asia-Pacific region and some parts of Africa. Melon fly attractants could improve the effectiveness of current pest management measures. Previous studies have shown that some host fruits are attractive to melon flies but few have investigated the chemical compounds responsible for their attraction.

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  • The study explored how gut microbiota changes in polyphagous insects during host transfers, specifically examining the dynamics of gut microbes in the fruit fly species *Bactrocera dorsalis* and its four different hosts.
  • Results indicated that the diversity of gut microbes decreased significantly during host transfer, influenced by the original host, the new host, and generations.
  • The findings suggest that gut microorganisms play a vital role in the adaptation of insects to new environments, offering insights for future pest control strategies.
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The fruit fly Zeugodacus tau (Diptera: Tephritidae) is a major pest of melons and other cucurbits in Southeast Asia. In this study, we used Illumina, Nanopore, and Hi-C sequencing technologies to assemble a reference genome of Z. tau at the chromosomal level.

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The pinion-streaked snout is a new potato pest that has recently been recorded in China. In this study, we analyzed the complete mitochondrial genome of . The results revealed the mitogenome (GenBank: OQ181231) to occur as a circular DNA molecule of 16,376 bp with 51.

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In recent decades, the increasingly widespread application of chemical pesticides has exacerbated the emergence of insecticide resistance among insect pests. In this study, we examined the rapid response of bacteria in the midgut of the fruit fly Bactrocera tau (Walker) (Diptera: Tephritidae) to stress induced by the insecticides lambda-cyhalothrin and spinosad by analyzing the bacterial community structure and diversity in the midguts of 4-day-old B. tau.

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Low temperatures greatly influence newly introduced species, and increased cold tolerance can facilitate their establishment in new environments. The invasive alien species is distributed at high latitudes and altitudes, where it suffers more from cold stress than it would at low latitudes or altitudes. Whether cold stress influences the accumulation of cryoprotectants and cold tolerance in , and further influences the cold tolerance of its biological control agent, , through feeding remain unknown.

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Article Synopsis
  • Next-generation sequencing was used to analyze the bacterial communities in the eggs and ovaries of the pest Bactrocera cucurbitae, which affects cucurbit fruits.
  • The study found that the egg microbiota exhibited greater diversity than the microbiota in the primary and mature ovaries, with 19 different bacterial phyla identified.
  • Key dominant phyla included Pseudomonadota and Bacteroidota, and understanding these microbial communities may help in developing strategies for managing this pest.
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Understanding tumor heterogeneity and immune infiltrates within the tumor-immune microenvironment (TIME) is essential for the innovation of immunotherapies. Here, combining single-cell transcriptomics and chromatin accessibility sequencing, we profile the intratumor heterogeneity of malignant cells and immune properties of the TIME in primary central nervous system diffuse large B-cell lymphoma (PCNS DLBCL) patients. We demonstrate diverse malignant programs related to tumor-promoting pathways, cell cycle and B-cell immune response.

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Seminal fluid proteins (SFPs) are key factors in sexual reproduction and are transferred to females during mating with sperm. SFPs have a nutritional value because they protect and activate sperm storage and release to optimize fecundity. Multiple matings promote ovipositioning in several insect species.

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The genus Fabricius, 1801 belongs to the tribe Galeritini of the family Carabidae. In this study, the complete mitochondrial genome (GenBank: ON920164.1) of .

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The genus Fischer von Waldheim 1827 belongs to the family Carabidae. Specimens of adult Liebke, 1931 were collected from Yājì Hill, Huáihuà City, Húnán Province, China. The complete mitochondrial genome (GenBank accession number ON674050.

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Systemic lupus erythematosus (SLE) is a chronic autoimmune disease, and CD4 T cells are known to promote SLE development. Here, we explore heterogeneities in the CD4 T cell regulome and their associations with SLE pathogenesis by performing assay for transposase-accessible chromatin with high-throughput sequencing (ATAC-seq) and single-cell transcriptome sequencing (single-cell RNA sequencing [scRNA-seq]) of peripheral CD4 T cells from 72 SLE patients and 30 healthy controls. Chromatin accessibility signatures of CD4 T cells are correlated with disease severity.

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Despite the significant progress that has been made in the genome sequencing of species, mitochondrial genome (mitogenome) used to examine genetic diversity within fungal populations. Complete mitogenomes of species can be easily sequenced and assembled using various sequencing techniques. However, since mitogenome annotations are mainly derived from similar species comparison and software prediction, and are not supported by RNA-seq transcripts data, it leads to problems with the accuracy of mitochondrial annotations and the inability to understand RNA processing.

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(Shelford, 1907) is bred as a pet because of its beautiful appearance. Its complete mitochondrial genome (GenBank accession number MW630139), obtained from the Manwan Town population, was characterized as the first complete mitogenome of the genus . The mitogenome consists of a circular DNA molecule of 16,627 bp with 76.

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To predict and mitigate the effects of climate change on communities and ecosystems, the joint effects of extreme climatic events on species interactions need to be understood. Using the common ragweed ( L.)-leaf beetle () system, we investigated the effects of heat wave and elevated CO on common ragweed growth, secondary metabolism, and the consequent impacts on the beetle.

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(Zhou, 2020) is widely distributed from central to eastern Yunnan with distinct geographical variations in morphology. Beetles were collected in Manwan Town, and a mitochondrial genome sequence (GenBank accession number MZ298928) of this species was sequenced using the MGI-SEQ 2000 platform, assembled using NOVOPlasty v4.3.

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Article Synopsis
  • The study explores how the cytokine storm in COVID-19, caused by the virus SARS-CoV-2, impacts patient mortality and identifies inflammatory responses shared with other diseases.
  • By analyzing single-cell transcriptomes from patients with COVID-19, sepsis, and HIV, researchers found ten hyperinflammatory cell subtypes, primarily driven by monocytes, that show different inflammation patterns in these infections.
  • The research proposes a "three-stage" model to categorize COVID-19 patients based on the inflammatory profiles of monocytes, aiming to enhance treatment strategies for various patient groups.
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The genus is a small arboreal group of darkling beetles that are endemic to the Oriental region. The complete mitochondrial genome of the population from the Manwan Town was first characterized. The mitogenome consisted of a circular DNA molecule of 15,662 bp with a 66.

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Complete mitochondrial genome (GenBank accession number MN073499) for (Amphibia: Caudata: Salamandridae) was obtained with Sanger sequencing and assembled manually. The mitogenome consists of a circular DNA molecule of 16,288 bp, with 68.51% AT content.

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