Fiber array-based large spot confocal Raman system for rapid in situ detection of pathogenic bacterial colonies.

Pathogenic bacteria infections are a major public health problem in current society. Rapid and reliable identification of these pathogens can help avoid the misuse of antibiotics and enable precision therapy. In this study, we present a large-spot confocal Raman system based on fiber array (LSCR-FA) for the in situ detection of microbial colonies on agar plates.

Siamese network for classification of Raman spectroscopy with inter-instrument variation for biological applications.

Raman spectroscopy has emerged as a highly sensitive, rapid, and label-free detection method, extensively utilized in biological research. Presently, it is frequently paired with artificial intelligence (AI) algorithms to facilitate identification and classification tasks. However, variations in the settings across different Raman spectrometers, along with the sensitive and continuous nature of biological Raman signals, can subtly alter the acquisition of these signals.

Raman cell sorting for single-cell research.

Cells constitute the fundamental units of living organisms. Investigating individual differences at the single-cell level facilitates an understanding of cell differentiation, development, gene expression, and cellular characteristics, unveiling the underlying laws governing life activities in depth. In recent years, the integration of single-cell manipulation and recognition technologies into detection and sorting systems has emerged as a powerful tool for advancing single-cell research.

Different genetic profiles contribute to worse overall survival in patients with leptomeningeal metastases of non-small-cell lung cancer.

Background: To assess the genetic characteristics of central nervous system (CNS) metastases from non-small-cell lung cancer (NSCLC), we gathered the genetic profiles of brain metastases (BM) and leptomeningeal metastases (LM). Our objective was to identify genetic factors contributing to poorer overall survival (OS) in NSCLC patients with LM.

Methods: This study included 25 consecutive patients with BM and 52 patients with LM from Guangdong Sanjiu Brain Hospital.

Precision isolation and cultivation of single cells by vortex and flat-top laser ejection.

Single-cell isolation stands as a critical step in single-cell studies, and single-cell ejection technology based on laser induced forward transfer technology (LIFT) is considered one of the most promising methods in this regard for its ability of visible isolating single cell from complex samples. In this study, we improve the LIFT technology and introduce optical vortex laser-induced forward transfer (OV-LIFT) and flat-top laser-induced forward transfer (FT-LIFT) by utilizing spatial light modulator (SLM), aiming to enhance the precision of single-cell sorting and the cell's viability after ejection. Experimental results demonstrate that applying vortex and flat-top beams during the sorting and collection process enables precise retrieval of single cells within diameter ranges of 50 μm and 100 μm, respectively.

An optimized DCO with modified binary-weighted DCTLs based hybrid tuning banks for an E-band DPLL.

An optimized millimeter-wave digital controlled oscillator (DCO) in a 40-nm CMOS process is presented in this work. The coarse-tuning modules and medium-tuning modules of the DCO utilize modified binary-weighted digitally controlled transmission lines (DCTLs) to achieve a better compromise among smaller chip size, higher resonant frequency, better tuning resolution and lower phase noise. The tuning precision and die size of the medium tuning bank are improved without changing the binary coding rules by replacing the lowest-weight bit of the DCTLs with switched capacitors.

[Retrospective study of the relationship of height difference and bone density of postmenopausal women].

Objective: To analyze the relationship of the height difference and bone density (BD) of premenopausal and postmenopausal women.

Methods: The height values of 191 premenopausal and postmenopausal women were recorded, and the BD values of lumbar vertebrae and hip were detected by double energy X-ray BD detector.

Results: The lower the height of the postmenopausal women, the less the BD value.